FORMOL TITRATION

NAME: WINDA A ENGKESA

NIM: 2015-78-064
Titration of formol is used to
show the level of Nitrogen in
amino, but it can also be used
to measure protein hydrolysis
FORMOL TITRATION OBJECTIVE
analysis of nitrogen content in amino by
means of formol titration to determine the
levels of protein available in the sample. in
formol titration, a solution containing a
protein made into an acid with an acidic
term titrated with a basic effect. The basic
solution used is NaOH. because NaOH is
included in the baking solution which is an
unstable trait in certain conditions it needs
to be standardized first by the nature of raw
PRINCIPLE OF FORMOL
• the protein solution is neutralized with a base of
sodium hydroxide (NaOH), then added
formaldehyde to form dimethylol
• with the formation of dimethillol the protein amino
protein group is bonded and does not affect the
reaction between the acid (carboxyl group) with
the NaOH base so that the end of the titration can
be precisely terminated
• indicator used is pp indicator the end of the
titration is marked when the pink color changes
are lost in 30 seconds
PRINCIPLE OF FORMOL
• formol titration is best used for evaluation of
the process of protein breakdown (eg in the
fermentation of proteins in tempeh, soy
sauce, and tauco)
• the process of protein hydrolysis is
characterized by increased formol titration
ANALSIS PROCEDURE

• take 10 ml or 10 g of sample into a 125 ml erlenmeyer and

add 20 ml of distilled water and 0.4 ml of saturated
potassium oxalate solution (potassium oxalate: water 1: 3)
and 1 ml of 1% phenolphthalein. let stand for 2 minutes
• sample the sample solution with 0.1 N sodium hydroxide
(NaOH) until it reaches a color such as a standard color or
pink color
• standard color: 10 ml of milk + 10 ml of distilled water +
0.4 ml of saturated potassium oxalate + 1 drops 0.01%
indicator rosanilin-chloride
• after the color has been achieved, add 2 ml of
40% formaldehyde solution and re-enrich it with
NaOH solution until color as standard color is
reached again. record this second titration

• make a blank titration comprising a comparison

of 20 ml of distilled water + 0.4 ml of saturated
potassium oxalate solution + 1 ml
phenolphthalein indicator + 2 ml formaldehyde
solution and titration with NaOH
CALCULATION FORMULA
% N = (TS-TB) x N NaOH x 14,008 x DF x 100%
sample weight (g) x 1000

TS= titration of the sample

TB= titration blank
SW= sample weight
DF= dilution factor = if according to the feeding
procedure DF = 50/10 = 5
REACTION TO FORMOL TITRATION
Advantages

Only precisely used to determine a process of

solving

disadvantages

Less precise to determine a particular type of

protein
Thank You