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UNIVERSITI
TEKNOLOGI
MARA
CENTRE OF GRADUATE STUDIES, FACULTY OF PLANTATION & AGROTECHNOLOGY

CHARACTERIZATION AND EVALUATION OF MUTANT

RICE LINES ML-3, ML-10 AND ML-30 UNDER WATER
STRESS TREATMENT
Student Name: Jeffry Shah bin Junus
Program : Mix mode MSc. AT 736 Plant Biotechnology
Supervisor: Dr. Shamsiah binti Abdullah

Date : 6th December 2017

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INTRODUCTION:
Our study’s main interest is to investigate the effects of ionic radiation induced
mutagenesis on rice lines and the performances of these mutant lines under
water stress conditions. To establish comparisons in our study, we have decided
to compare the ionic radiation mutant rice lines with two other rice lines; first the
check variety and the second, are gamma radiation induced mutant lines.

PROBLEM STATEMENTS:
Ionic radiation was used to produce mutant rice lines which was hypothesized to
have higher genetic diversity. The increase of genetic diversity in mutant rice
lines were predicted to yield significant cultivar improvements and the research
assess any potential increase in performances of the rice lines under severe
water stress conditions.

OBJECTIVES:
1. To assess the morphological and agronomic trait improvements of mutant
rice lines ML-3, ML-10 and ML-30 under severe water stress conditions.
2. To assess the genetic diversity of the mutant rice lines via ISSR markers
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METHODOLOGY
(State the standard methodology employed in this study. It must be a standard
method or please state appropriate references)
No Methodology References

Obj. 1 1. Flag Leaf Lengths and Widths (cm) Application of Mutation and
Biotechnology for Minimal Water
2. Culm Lengths and Culm Number (cm) Requirement and Improvement of
3. Number of Tillers per plant Amylose Content in Rice, (Ibrahim et
al., 2010)
4. Panicle Lengths (cm) and Number of Panicles
per Plant Evaluation and Characterization of
Advance Mutant Rice Lines of Rice
5. Plant Height (cm) MR219-4 and MR219-9 under
6. Days to maturity Drought Conditions, (Abdul Rahim et
al., 2012)
7. 100- Grain Weights (g)
8. Grain Weight per Plant (g)

Obj. 2 1. ISSR gene markers

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MILESTONES
(State the percentage of objectives achieved in this study)
No Literature Methodology Data & Results & Overall
Analysis Discussion Comments

Obj. 1 50 % 50% 50% 30%

Obj. 2 50% 50% 0% 0%

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MAJOR PROBLEMS
1. Delay in the delivery of essential items for the experiment.
2. Pest problems in UiTM Puncak Alam Rain Shelter

CONCLUSION
Overall progress of research is slow.
The expected date of experiment completion is in January 2018.
The expected date of thesis completions is in February 2018.
The expected date of thesis submission is in March 2018.