Its quiz time!

Boggusrl@email.uc.edu
 How Rachel is going to do
this
• Put up a slide
• Write down the answer
• Go over it immediately
• Repeat many many times
– 113 slides of adventure await!!

Did you remember your party hats????

What type of epithelium is this?
(how do you know?)
 Transitional Epithelium
• How do you know?
– Scalloped appearance
– Bi-nucleated cells
What is the predominant type
of tissue? (how do you know?)
 Smooth Muscle
• How do you know?
– Nuclei more centrally located
– Seen here in both planes of section
– In cross section you can tell its smooth muscle
because of the centrally located nuclei but
also because some cells do not have the
nucleus cut in the plane so look anucleate
– Two orientations of the tissue (longitudinal and
cross section)
 What is this?
How do you know?
 Peripheral Nerve
• How do you know?
– Encased by an perineurium
– Wavy/bubbly appearance of the nerve
fibers within perineurium

REMEMBER: CIRCLE OF WAVY SHIT

What is the arrow pointing
to?
 Serous Demilune
• How did you know?
– Because most of it looks like a mucous
secreting gland (vacuolated cytoplasm
with laterally displaced nuclei) But the
crescent shaped rim (hence
LUNE=Moon!) is more like a serous
gland (basophilic) so it’s a serous
demilune
What is this? How do you
know?
 Dense Irregular Connective
Tissue
• How do you know?
– Few nucleated cells - fibroblasts
– Lots of collagen arranged random-like
– Nuclei located outside of the collagen
What is the predominant
tissue?
 Brown (multilocular) fat
• How do you know?
– Bubbly appearance signifies many fat-
filled vesicles
– Nuclei still laterally displaced
– Remember that you have multilocular
fat as a stage of differentiation to
regular white fat.
What type of tissue?
how do you know?
 Fibrocartilage
• How did you know?
– No perichondrium evident
– Cells located in lacunae
What process is occurring in
this slide?
 Intramembranous Bone
Formation
• How did you know?
– Osteoblasts surrounding osteoid with
osteocytes enclosed in the osteoid…
What type of epithelium?
 Ciliated pseudostratified
columnar epithelium

• How did you know?

– Because Dr. Michaels drilled this into
your brains?
• NO! (well ok, probably) but also because
you can sort of see that all the cells
maintain their connection with the basal
lamina and you can clearly see cilia
What is the tissue?
 Cardiac muscle
• How did you know?
– Centrally located
nuclei surrounded by
large ring of
cytoplasm
– For the most part,
more uniformly
shaped cuts
– Compare to smooth
muscle cut in cross
section – smooth has
smaller muscle fibers
and nuclei bigger in
relation to size of fiber
 Which of the following are
Transmission Electron
micrographs? And what are the
EMs of?
C.
A.

D.
B.
 A, C, D
• REMEMBER THAT FREEZE FRACTURE EMS
ARE VIEWED WITH TRANSMISSION EM!
• All of them are cillia
– Remember the basal bodies (what is the
sydrome when you don’t have dynein
arms?
• Kartageners syndrome
• What was used to obtain image B?
– Scanning EM
What is indicated by the red
arrow?
 Glycogen
• How did you know
– Because that is what
glycogen looks like on the
EM
– Compare to collagen cut in
cross section
– Remember that glycogen
particles aggregate and
also that they exist OVER
other stuctures, usually SER
– Glycogen intracellular,
collagen EXTRAcellular
What is this?
 Golgi Apparatus
• Layered like apperance
• Which end is trans/cis?
What is this?
 ganglion
• How did you know?
– Bullseye like appearance of the cells.

FRIED EGGS
What are indicated by the
green arrows?
 Osteoclasts
• How did you know?
– Multinucleated cells
– Found in indented areas of bone (know
its bone because you can see the
osteocytes in lacunae (yellow arrows)
 Ignore the arrow
What is predominate on this
slide?
 Serous glands
• How did you know?
– Because it looks
glandular and
there is still color in
the cytoplasm.
Compare to
mucous secreting
glands, which
have vacuolated
cytoplasm
What is indicated by the
number 1? 2?
 1) Bone Marrow 2)
spongy/cancellous bone
spicule
• How do you know?
– 1) you know it is bone marrow because it is
found between trabeculae of bone (bright
red) and it is rich in lipid and hematopoetic
cells (purple) Ok, so you haven’t had this yet,
I’m just preparing you for next block.
– 2) you know its cancellous bone because you
can see the osteocytes in lacunae
 Label A-D
____A__________B___________C____________D______
• A) Zone of resting cartilage
• B) Zone of proliferation
• C) Zone of hypertrophy
• D) Zone of calcification
• What process was indicated?
– Endochondrial bone formation
What is along the lumen?
 stereocilia
• You know because it branches and
it is long
• Another clue – stereocilia often in
epididymis. You don’t know what
this looks like yet but it is like
35148743 small tubes like this.
ALWAYS stereocilia here
Epididymis far away
What is this?
 Loose Irregular Connective
Tissue
• You know because there is a
general lack of nuclei and very
sparse and randomly placed
collagen
What is indicated by the
arrow?
 Mast Cells
• You can tell because it is purple
because of metochromasia
• And if you look really closely you
can see that it is a granule-filled cell.
What is this?
 Dense Regular Connective
Tissue
• How did you know?
– Wavy appearance of collagen
– Few cells (fibroblasts) located outside
of the collagen fibers
What is indicated by the
arrow?
 Node of Ranvier
• You know because it is stained for
myelin and the indentation  place
without myelin
• What does myelin do?
• What forms it?
What is indicated by the red
and blue arrows?
 Blueepineurium
Redperineurium
• You just need to remember that epi
is outside, peri is middle and endo is
innermost… remember this for both
nerves and muscles.
What is the epithelium?
 Non-keratinized stratified
squamous epithelium
• How did you know?
– Because the cells get
flattened as they get close
to the lumen
– Non-keratinized because
there is not layers of
anucleated cells on the
surface (see pictures)
What is indicated at 1,2, 3, what is
the whole thing called in EM, light
microscopy?
Did someone say “bar”????
• 1) zonula occludens
• 2) zonula adherens
• 3) macula adherens or desmosome
• In EM the three together
Junctional complex
• In light microscopy terminal bar
What is this?
 Mast cell
• You know because of the dark
granules in the cytoplasm
• What are the contents of the cell?
– Histamine and heparin
What are indicated by the
arrow?
 Centrioles
Near nucleus somewhat

Participate in cell division

What stage of cell division is
indicated by the arrow?
 Metaphase
• You know because the
chromosomes are lined up at the
center of the cell and you can see
the spindle fibers extending from
them.
What is indicated by #5
 Goblet cell
• What would it look like under the
light microscope?
– Vacoulated space, maybe erupting
• How would you make it stand out
more?
– Stain with PAS
What is indicated by the
arrows
 Basement membrane
• REMEMBER it is called a basement
membrane under light microscopy
but under EM you can distinguish the
different parts!
• What is the epithelium?
– Ciliated pseudostratified columnar
epithelium with goblet cells =)
What is the epithelium?
 Simple cuboidal

• How do you know?

– Because the nuclei are
centrally located within
the cytoplasm
– Compare with simple
columnar in which the
nuclei are located
more to one end of the
cell
What is this arrow pointing
to?
Well it’s a mitochondria

whats in the mito?

Mg and Ca granules!
Whats all this business?
 SER
• Bubbly looking
• Spread randomly throughout cell
• Not flat pancakes like RER

• What do we often see with SER?

– Round mito and glycogen
This is?
 karyotype
If you forget this….

Well, just don’t be that guy

•What’s
this?
 Your mom!
• No, really it’s a secondary lysosome
• Has a bunch of random stuff in it,
heterogeneous, if you will
– You must say secondary or else no
points for you
Whats the arrow pointing
to?
 melanin aka pigment
• Why? Under the epidermis
• Brown stuff in it
Green arrow?
 heterochromatin
• 1. In nucleus
• 2. dark
 What cytoskeletal element
is the red arrow pointing to?
 microtubule
• 3 kinds involved in mitotic apparatus
– 1. Ones that go across
– 2. Astral ones that radiate out
– 3. Ones attached to chromosomes,
can’t see these
Whats this? How do you
know??
 dendrite
• Why not axon?
• 1. Because I said so
• 2. Has nissl substance – this is KEY
difference
Whats on the left?
 polysomes
• Swirls of ribosomes

• Do not mistake for grainy glycogen

or collagen fibers – neither make
swirls
Whats this tissue?
 Mesenchyme!
• Cells all look the exact same
• Absolutely no pattern
• Ground substance looks same in the
background throughout whole thing
Black fibers are….?
 Reticular fibers
• Usually have to use the black stain
• Tree branches as opposed to
collagen and elastin that go in
straight lines across the slide
Name it
 Elastic cartilage
• Can see nice little fibers
• perichondrium
 And the survey says….
• Green = haversian CANAL (h. system
is the osteon. Do not mix up)
• Yellow = interstitial lamellae
– Anything that isn’t part of an osteon
Red and yellow?
Red = perichondrium:
know its hyaline, looks
acellular

Yellow = isogenous
groups/nests.
Predominant tissue?
 Skeletal muscle
• Biggest cells when cut in cross
section
• Little baby nuclei all off one one side
And this is?
 Smooth muscle. X section.
• Only a little space between cells
• Nuclei not in every one
?????
 Neuromusular jxn aka
motor end plate
• See skeletal muscle
• See nerve on skeletal muscle
And these?
 Myelinated nerves
• Remember, if you don’t write myelin
you will die
• Black stuff wrapped around nerve
axon
And the green arrows is
pointing to?
 collagen
Very regular looking when cut in X
section. Perfect circles vs swirls for
polysomes and rough looking stuff
for glycogen
 mitochondria
• Lots of it with muscle because they
need lots of E
• Glycogen too
 What dye is being picked
up by the thing that the
yellow arrow is pointing to?
 eosin
• As opposed to……
– Hematoxylin which is purple
– PAS which is MAGENTA and stains the
Gs
• Glycogen
• glycoprotein
• glycocalyx
• Golgi
Whats 1? Whats 2?
2 = granular portion of
nucleolus – looks more
grainy
1 = fibrillar portion of
nucleolus
 THE END

boggusrl@email.uc.edu