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Boggusrl@email.uc.edu
How Rachel is going to do
this
• Put up a slide
• Write down the answer
• Go over it immediately
• Repeat many many times
– 113 slides of adventure await!!
D.
B.
A, C, D
• REMEMBER THAT FREEZE FRACTURE EMS
ARE VIEWED WITH TRANSMISSION EM!
• All of them are cillia
– Remember the basal bodies (what is the
sydrome when you don’t have dynein
arms?
• Kartageners syndrome
• What was used to obtain image B?
– Scanning EM
What is indicated by the red
arrow?
Glycogen
• How did you know
– Because that is what
glycogen looks like on the
EM
– Compare to collagen cut in
cross section
– Remember that glycogen
particles aggregate and
also that they exist OVER
other stuctures, usually SER
– Glycogen intracellular,
collagen EXTRAcellular
What is this?
Golgi Apparatus
• Layered like apperance
• Which end is trans/cis?
What is this?
ganglion
• How did you know?
– Bullseye like appearance of the cells.
FRIED EGGS
What are indicated by the
green arrows?
Osteoclasts
• How did you know?
– Multinucleated cells
– Found in indented areas of bone (know
its bone because you can see the
osteocytes in lacunae (yellow arrows)
Ignore the arrow
What is predominate on this
slide?
Serous glands
• How did you know?
– Because it looks
glandular and
there is still color in
the cytoplasm.
Compare to
mucous secreting
glands, which
have vacuolated
cytoplasm
What is indicated by the
number 1? 2?
1) Bone Marrow 2)
spongy/cancellous bone
spicule
• How do you know?
– 1) you know it is bone marrow because it is
found between trabeculae of bone (bright
red) and it is rich in lipid and hematopoetic
cells (purple) Ok, so you haven’t had this yet,
I’m just preparing you for next block.
– 2) you know its cancellous bone because you
can see the osteocytes in lacunae
Label A-D
____A__________B___________C____________D______
• A) Zone of resting cartilage
• B) Zone of proliferation
• C) Zone of hypertrophy
• D) Zone of calcification
• What process was indicated?
– Endochondrial bone formation
What is along the lumen?
stereocilia
• You know because it branches and
it is long
• Another clue – stereocilia often in
epididymis. You don’t know what
this looks like yet but it is like
35148743 small tubes like this.
ALWAYS stereocilia here
Epididymis far away
What is this?
Loose Irregular Connective
Tissue
• You know because there is a
general lack of nuclei and very
sparse and randomly placed
collagen
What is indicated by the
arrow?
Mast Cells
• You can tell because it is purple
because of metochromasia
• And if you look really closely you
can see that it is a granule-filled cell.
What is this?
Dense Regular Connective
Tissue
• How did you know?
– Wavy appearance of collagen
– Few cells (fibroblasts) located outside
of the collagen fibers
What is indicated by the
arrow?
Node of Ranvier
• You know because it is stained for
myelin and the indentation place
without myelin
• What does myelin do?
• What forms it?
What is indicated by the red
and blue arrows?
Blueepineurium
Redperineurium
• You just need to remember that epi
is outside, peri is middle and endo is
innermost… remember this for both
nerves and muscles.
What is the epithelium?
Non-keratinized stratified
squamous epithelium
• How did you know?
– Because the cells get
flattened as they get close
to the lumen
– Non-keratinized because
there is not layers of
anucleated cells on the
surface (see pictures)
What is indicated at 1,2, 3, what is
the whole thing called in EM, light
microscopy?
Did someone say “bar”????
• 1) zonula occludens
• 2) zonula adherens
• 3) macula adherens or desmosome
• In EM the three together
Junctional complex
• In light microscopy terminal bar
What is this?
Mast cell
• You know because of the dark
granules in the cytoplasm
• What are the contents of the cell?
– Histamine and heparin
What are indicated by the
arrow?
Centrioles
Near nucleus somewhat
Mg and Ca granules!
Whats all this business?
SER
• Bubbly looking
• Spread randomly throughout cell
• Not flat pancakes like RER
Yellow = isogenous
groups/nests.
Predominant tissue?
Skeletal muscle
• Biggest cells when cut in cross
section
• Little baby nuclei all off one one side
And this is?
Smooth muscle. X section.
• Only a little space between cells
• Nuclei not in every one
?????
Neuromusular jxn aka
motor end plate
• See skeletal muscle
• See nerve on skeletal muscle
And these?
Myelinated nerves
• Remember, if you don’t write myelin
you will die
• Black stuff wrapped around nerve
axon
And the green arrows is
pointing to?
collagen
Very regular looking when cut in X
section. Perfect circles vs swirls for
polysomes and rough looking stuff
for glycogen
mitochondria
• Lots of it with muscle because they
need lots of E
• Glycogen too
What dye is being picked
up by the thing that the
yellow arrow is pointing to?
eosin
• As opposed to……
– Hematoxylin which is purple
– PAS which is MAGENTA and stains the
Gs
• Glycogen
• glycoprotein
• glycocalyx
• Golgi
Whats 1? Whats 2?
2 = granular portion of
nucleolus – looks more
grainy
1 = fibrillar portion of
nucleolus
THE END
boggusrl@email.uc.edu