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Opening of zona
Transfer of spindle Separation of
pellucida using
Membrane Fusion to perivitelline spindle from
LYKOS Laser
space cytoplasm
System
Culture in Global
Injection of single Total Medium Blastocyst
Incubation
motile sperm (replaced every 48 formation
hours)
RESULTS & DISCUSSION
• Successful reconstitution and exposure to intracytoplasmic sperm of
oocytes
• Four out of five fertilized and developed into blastocysts
• One out of four embryos euploid, three aneuploid
• Comparison of mtDNA sequence of mother, donor, and embryo
showed an average transmission rate of 5.10±1.11%
• Transfer of euploid embryo led to uneventful pregnancy
• Genotyping of baby expressed a normal karyotype
CONCLUSION
• Spindle transfer may minimize the transmission of mutant mtDNA
from a carrier mother to her child.
• MST accomplished carryover rate of less than 6%.
• As of 7 months, baby is still asymptomatic.