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Tiana Milanda
Outline
• Anatomy & Functions of Lymphoid
Tissues & Organs
Figure 1-3
1. Originated from
Bone Marrow (BM)
2. Two major
lineages: Thymus
- Lymphoid
- Myeloid
Effector cells APCs
B cell development in the
Bone Marrow
Granule
Mast cell
1 IgE antibodies produced in On subsequent exposure to the 3 Degranulation of the cell,
response to initial exposure same allergen, IgE molecules triggered by cross-linking of
to an allergen bind to attached to a mast cell recog- adjacent IgE molecules,
receptors or mast cells. nize and bind the allergen. releases histamine and other
chemicals, leading to allergy
symptoms.
Granulocytes
• Neutrophils
– Contain alkaline phosphatase, lysozyme, lactoferrin,
phagocytin and type IV collagenase
– Highly phagocytic and motile
– Leave blood and enter infected tissue
– The earliest phagocytic cells to appear in a bacterial
(extracellular pathogens) infection and are
prominent constituent of pus
Granulocytes
• Eosinophils
– Contain histaminase, acid phosphatase and major
basic protein
– Somewhat phagocytic
– Ability to leave blood
– Destroying large parasites (helminths),
phagocytosing antigen-antibody complexes and
combating histamine levels during allergic reactions
Agranulocytes
• Agranulocytes
– Granules not visible after staining
– 2 types
• Monocytes – phagocytic leukocytes in blood
Macrophage – monocyte that has entered tissue and
has matured
• Lymphocytes (T and B) and NK cells
Monocytes
Monocytes :
– Leave circulation to give rise to macrophages within
almost every organ
Macrophages :
– Contain azurophilic granules (lysozomes)
– More phagocytic than neutrophils or esinophils
degrade larger bacteria within phagosomes via
formation of hydrogen peroxide,hypochlorus acid and
superoxide
– Macrophages and dendritic cells express Class II
MHC (major histocompatibility complex) and can
function as APC (antigen presenting cells) . They
secrete cytokines and tumor necrosis factor
Maturation of Macrophages
Activated Macro
Dendritic cells as
Ag-presenting cells
Classes of
Lymphocytes
1. NK cells
2. T cells:
- T helper cells
- T cytotoxic cells
- T regulatory cells
=> suppress immune
responses
1. NK cells
2. T cells:
- T helper cells
- T cytotoxic cells
- T regulatory cells
=> suppress immune
responses
Figure 21.15a
The activated
cytotoxic
T cell
1 A specific cytotoxic T cell binds to a 2 The activated T cell releases perforin 3 The granzymes initiate apoptosis within the
class I MHC–antigen complex on a molecules, which form pores in the target cells, leading to fragmentation of the
target cell via its TCR with the aid of target cell membrane, and proteolytic nucleus, release of small apoptotic bodies,
CD8. This interaction, along with enzymes (granzymes), which enter the and eventual cell death. The released
cytokines from helper T cells, leads to target cell by endocytosis. cytotoxic T cell can attack other target cells.
the activation of the cytotoxic cell.
Target
cell Peptide
antigen Cytotoxic
T cell
Class II MHC molecules
• Located mainly on dendritic cells,
macrophages, and B cells
– Display antigens Microbe Antigen-
presenting
1 A fragment of
to helper T cells foreign protein cell
Antigen
(antigen) inside the
cell associates with fragment
an MHC molecule
and is transported 1
to the cell surface.
Class II MHC
2 molecule
2 The combination of T cell
MHC molecule and receptor
antigen is recognized
by a helper T cell, alerting it
to the infection.
Helper T cell
(b)
Class II MHC Proteins
Figure 21.15b
The role of helper T cells
in acquired immunity
1 After a dendritic cell engulfs and degrades a bacterium, it displays
bacterial antigen fragments (peptides) complexed with a class II
MHC molecule on the cell surface. A specific helper T cell binds
to the displayed complex via its TCR with the aid of CD4. This
interaction promotes secretion of cytokines by the dendritic cell.
Cytotoxic T cell
Dendritic Peptide antigen
cell Helper T cell Cell-mediated
Class II MHC
Bacterium molecule immunity
(attack on
TCR infected cells)
2 3
Humoral
1 CD4 immunity
(secretion of
Dendritic Cytokines B cell antibodies by
cell plasma cells)
2 Proliferation of the T cell, stimulated 3 The cells in this clone
by cytokines from both the dendritic secrete other cytokines
cell and the T cell itself, gives rise to that help activate B cells
a clone of activated helper T cells and cytotoxic T cells.
(not shown), all with receptors for the
same MHC–antigen complex.
Classes of
Lymphocytes
1. NK cells
2. T cells:
- T helper cells
- T cytotoxic cells
- T regulatory cells
=> suppress immune
responses
Peptide
antigen
Class II B cell
MHC
molecule 2 Secreted antibody
3 Clone of plasma cells
1 molecules
TCR CD4 Endoplasmic
reticulum of
plasma cell
Cytokines
• Immunoglobulin A (IgA)
Promotes opsonization, neutralization, and agglutination
of antigens; less effective in complement activation than
IgM (see Figure 43.19)
• Immunoglobulin E (IgE)
Figure 43.19)
IgE
(monomer) Triggers release from mast cells and basophils of
histamine and other chemicals that cause allergic
reactions (see Figure 43.20)
• Immunoglobulin A (IgA)
Promotes opsonization, neutralization, and agglutination
of antigens; less effective in complement activation than
IgM (see Figure 43.19)
• Immunoglobulin E (IgE)
Figure 43.19)
IgE
(monomer) Triggers release from mast cells and basophils of
histamine and other chemicals that cause allergic
reactions (see Figure 43.20)
2. Cell-mediated immunity
=> Key player => T cells =>
regulate other immune
cells
=> Target intracellular
microbes, e.g. viruses,
bacteria