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Evaluation of Anti-Quorum Sensing Activity of Methanolic

Leaf Extract of Lantana camara (Kantutai) against


Staphylococcus aureus
and Pseudomonas aeruginosa
Shane Valerie G. Bautista1,2,3, Aira Shayne R. Bituin1,2,3, Nikki Jean P. Hechenagocia1,2,3, Sheryl M. Manzano1,2,3,
Rhenzie Philip L. Mendoza1,2,3, Jewel Lynn S. Salonga1,2,3, Mariel Nina M. Esguerra, RPh1,2,3,4, April B. Lingat
RPh1,2,3,4

College of Pharmacy1, Research and Development and Innovation Center2Our Lady of Fatima University3,
Research Advisers4
WHAT IS QUORUM
SENSING?
Lantana camara
What are the phytochemical constituents

1 present in the methanolic leaf extract of


Lantana camara (Kantutai)?

2 What are the functional groups present in


the methanolic leaf extract of Lantana camara
(Kantutai)?

At what concentration of the methanolic


STATEMENT OF 3 leaf extract of Lantana camara (Kantutai) will
exhibit anti-quorum sensing activity?
THE PROBLEM Is there a significant difference between the anti-

4 quorum sensing property of leaf methanolic extract of


Lantana camara (Kantutai) with the positive
(Chloramphenicol)?

5 Is there a significant difference between the anti-


quorum sensing property of the methanolic leaf
extract of Lantana camara (Kantutai) with the
negative control (distilled water)?
Collection

Drying

Maceration

Phytochemical FTIR analysis Bioassay


Screening

Twitching Swarming

(+) Control = Chloramphenicol

(-) Control = Distilled Water

25% Methanolic Leaf Extract

METHODOLOGY OF 50% Methanolic Leaf Extract

75% Methanolic Leaf Extract


THE STUDY
Procedure for Twitching Assay Procedure for Swarming Assay
INCUBATE
TRANSFER THEN MIX

INOCULATE

TRANSFER

TRANSFER INOCULUM
STAB

INCUBATE
INCUBATE
MEASURE MEASURE
Obtained extract = 1.2 g

DILUTION OF METHANOL
1 g of extract = 10 mL of methanol

1.2 g of extract * 10 mL of methanol = 12mL

12 mL/3 conc = 4 mL

4 mL * 0.25 = 1 mL extract + 3 mL distilled water

PREPARATION OF THE 4 mL * 0.50 = 2 mL extract + 2 mL distilled water


PLANT EXTRACT 4 mL * 0.75 = 3 mL extract + 1 mL distilled water
CONCENTRATION
PHYTOCHEMICAL SCREENING
RESULTS
FTIR RESULTS Constituents Methanol

ALKALOIDS
-
AMINO ACIDS
3356.78 -
Alcohol / Phenol O-H Stretch CARBOHYDRATES
+
FIXED AND FATS OIL
-
GLYCOSIDES
+
1647.78 PHENOLIC COMPOUNDS AND
+
Amide C=O Stretch & Alkenyl C=C Stretch TANNINS
1637.83
PHYTOSTEROL
-
PROTEIN
-
1558.36 SAPONIN
-
1542.11 Aromatic C=C Stretch
1507.60 GUM AND MUCILAGES
-
VOLATILE OIL
-
Results of Twitching Assay of Pseudomonas aeruginosa Results of Swarming Assay of Pseudomonas aeruginosa
Pseudomonas aeruginosa Pseudomonas aeruginosa
Plates no. (+) Control (-) Control 25% 50% 75% Plates no. (+) Control (-) Control 25% 50% 75%
1 0.7 cm 1.4 cm 1.1 cm 0.8 cm 0.9 cm 1 2.2 cm 5.3 cm 1.9 cm 2.1 cm 1.9 cm
2 0.6 cm 1 cm 0.5 cm 0.6 cm 0.5 cm 2 3.1 cm 4.2 cm 1.5 cm 1.6 cm 1.6 cm
3 0.6 cm 1 cm 1.2 cm 1.1 cm 0.5 cm 3 3.3 cm 4.1 cm 3.1 cm 1.8 cm 1.7 cm
Mean 0.63 cm 1.13 cm 0.93 cm 0.83 cm 0.63 cm Mean 2.87 cm 4.53 cm 2.17 cm 1.83 cm 1.73 cm

Results of Twitching Assay of Staphylococcus aureus Results of Swarming Assay of Staphylococcus aureus
Staphylococcus aureus Staphylococcus aureus
Plates no. (+) Control (-) Control 25% 50% 75% Plates no. (+) Control (-) Control 25% cm 50% 75%
1 0.50 cm 0.60 cm 1.70 cm 1.10 cm 0.60 cm 1 1.6 cm 2.6 cm 2.6 cm 2.5 cm 2.4 cm
2 0.45 cm 0.90 cm 0.60 cm 0.50 cm 0.61 cm 2 1.2 cm 2.7 cm 2.7 cm 2.4 cm 2 cm
3 0.50 cm 0.90 cm 0.80 cm 0.80 cm 0.55 cm 3 1.3 cm 3.1 cm 3.1 cm 2.6 cm 2.2 cm
Mean 0.48 cm 0.80 cm 1.03 cm 0.80 cm 0.59 cm Mean 1.03 cm 2.8 cm 2.8 cm 2.5 cm 2.2 cm
CONCLUSION
The conclusion of this study was based on the different experimental analysis that
was done by the researchers.

1. Upon conducting the Phytochemical screening of the methanolic leaf extract of Lantara
camara (Kantutai), glycosides, carbohydrates and phenolic compounds and tannins are
obtained as constituents.
2. Based on the Fourier Transport Infrared Spectroscopy analysis, alcohol / phenol, amide and
alkenyl, and aromatic ring are found.
3. The methanolic leaf extract of Lantana camara (Kantutai) exhibit anti–quorum sensing
activity at the concentration of 75% against the said species Pseudomonas aeruginosa and
Staphylococcus aureus.
4. There is no significant difference between the methanolic leaf extract of Lantana camara
(kantutai) with the positive control chloramphenicol in the inhibition of qouum-sensing activity.
5. There is a significant difference between the methanolic leaf extract of Lantana camara
(kantutai) with the negative control distilled water in the inhibition of quorum-sensing activity.

Therefore the methanolic leaf extract of Lantana camara (kantutai) exhibit anti–quorum
sensing activity against the species of Pseudomonas aeruginosa and Staphylococcus aureus.
Results of Twitching Assay of Pseudomonas aeruginosa
Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value

Positive vs. 25% 0.1675 -0.3157 to 0.6507 No ns 0.7327 A-C


Positive vs. 50% 0.335 -0.1482 to 0.8182 No ns 0.2183 A-D
Positive vs. 75% 0.585 0.1018 to 1.068 Yes * 0.0171 A-E
Results of Swarming Assay of Pseudomonas aeruginosa

Significant
Mean Diff. 95.00% CI of diff. ? Summary Adjusted P Value

Positive vs. 25% 0.7 -0.1476 to 1.548 No ns 0.1183 A-C


Positive vs. 50% 1.035 0.1874 to 1.883 Yes * 0.0162 A-D
Positive vs. 75% 1.135 0.2874 to 1.983 Yes ** 0.0089 A-E
Results of Twitching Assay of Staphylococcus aureus
Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value

Positive vs. 25% -0.55 -1.078 to -0.02242 Yes * 0.0403 A-C


Positive vs. 50% -0.3175 -0.8451 to 0.2101 No ns 0.3216 A-D
Positive vs. 75% -0.105 -0.6326 to 0.4226 No ns 0.9318 A-E
Results of Swarming Assay of Staphylococcus aureus

Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value

Positive vs. 25% -1.518-1.88 to -1.155 Yes **** <0.0001 A-C


Positive vs. 50% -1.218-1.58 to -0.8547 Yes **** <0.0001 A-D
Positive vs. 75% -0.9175-1.28 to -0.5547 Yes **** <0.0001 A-E
RECOMMENDATION

After the experimentation was done the following


recommendations are offered for related research in the field of
pharmacy and bacteriology

1. To conduct further studies about the anti-quorum sensing


activity of Lantana camara
2. To formulate a dosage form that is befit for the plant extract
3. To further develop another study using other parts of Lantana
camara
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