CARBOHYDRATES

C L I N I C A L C H E M I S T RY I
L A B O R AT O R Y
BASIC BIOCHEMISTRY
BASIC BIOCHEMISTRY
BASIC BIOCHEMISTRY
BASIC BIOCHEMISTRY
BASIC BIOCHEMISTRY
SPECIMEN
FASTING
CHEMICAL METHODS OF
DETERMINATION
• Copper reduction methods
– Nelson-Somoygi
– Folin-Wu
– Neocuprine
CHEMICAL METHODS OF
DETERMINATION
• Ferric reduction methods
– Hagedorn-Jensen
 CHEMICAL METHODS OF
DETERMINATION
• Condensation methods (Dubowski)

• Glucose + O-toluidine + Acetic Acid + 100°C  Bluish-green complex

ENZYMATIC METHODS
• Glucose oxidase
ENZYMATIC METHODS
• Hexokinase
OTHER METHODS OF GLUCOSE
ASSESSMENT
• OGTT
GLYCATED HEMOGLOBIN
FRUCTOSAMINE
KETONES
DIABETES