SEED CONSERVATION BIOLOGY OF WILD SPECIES OF INDIAN MUSA AND DEVELOPMENT OF CRYOPRESERVATION PROTOCOLS

Presented By
Shivani
Enrolment No: A0530716020
Amity Institute of Biotechnology
External Guide
With the Guidance of Dr. Anuradha Agrawal
Dr. Rajeev Kumar Principal Scientist
Assistant Professor Tissue Culture and Cryopreservation Unit
Amity Institute of Biotechnology ICAR-National Bureau of Plant Genetic
Amity University Uttar Pradesh Resources, Pusa Campus
Sector-125, Noida-201313, U.P. New Delhi – 110 012, India
 Objectives
•To investigate the seed conservation biology of wild species of Musa (especially M.
balbisiana) to determine its storability,germination and viability

• Development of protocols for cryopreservation of wild Indian Musa species, using

seed, embryo and meristem explants

• Conservation of germplasm of wild Indian Musa species, based on above-optimized

methods

2
 Work done in current semester
 Germplasm collection of three different genome
Germination % Germination % 100

group (M. balbisiana (BB), M. ssp.(AB) and M. Time after storage without storage 90

80

acuminata (AA)) from Dept. of Biotechnology, 24 hrs 83.03 85.71 70

60

48 hrs 91.96 86.67

G ermin atio %n
Mizoram University. 50

40

 Experiments on seed storage for different duration at 72hrs 80.67 80.56 30

20

-20ºC to check the freezing senstivity of the seeds on 7 days 86.68 79.16 10

0
24 hrs 48 hrs 72hrs 7 days 1 month 3 months 6 months
exposure to low temperature w.r.t mc.(Table.1.& 1 month 82.89 84.72 Different time durations of storage at -20 ° after storage
without storage

Fig.1.) 3 months 86.68 90.27

 After statistical analysis through SPSS-17 software, 6 months 93.88 90

germination after storage w.r.t before storage doesn’t Table: Germination % before and after Fig.1. Germination % of embryos , before and
storage at -20°C after storage at -20°C.
have any significant differences(0.005), thus the
seeds are freezing insensitive (and therefore not
recalcitrant) and can be store at -20ºC for upto 6
months with ~10% mc (according to mentioned A B
A B
data).(Fig.2. & Fig.3.)
 Ongoing experiments for breaking seed coat
dormancy by acid treatment(Sulphuric acid) in
C D
C D
different concentrations for different durations. Fig.3. A. & B: Germination before storage ; C. &
Fig.2. Different stages of embryo growth D: Germination after storage
 Achievements / Plan for next semester
Achievements:
Attended ICGEB Workshop on “Plant responses to light and stress: Emerging issues in
climate change” from October 10-12, 2018 at ICGEB, New Delhi, India.
Collected different genomic groups of wild Musa species from Mizoram University

Plan for next semester:

Experiments on ageing of seeds to know about the maximum viability of seeds/embryos to
characterise the type of seeds.
 Biochemical analysis of seeds to know about the constituents which play major role in their
germination and storage.
Experiments on breaking seed coat dormancy to check the germination of whole seed in in
vitro conditions