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  • Introduction To HPLC December 2016

    Transféré par

    Robert Coffin
    17 vues8 pages
    The document is an introduction to high performance liquid chromatography (HPLC) presented by Robbie Coffin of Industrial Microbiological Services Limited (IMSL). It provides a brief history of HPLC, explaining it was developed in the early 20th century by Russian scientist Mikhail Tswett. The document defines HPLC and describes the basic components of an HPLC system. It also outlines the main modes of HPLC including normal phase, reverse phase, size exclusion, and ion exchange. Finally, it states that HPLC services are provided by IMSL.

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    hplc

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    Introduction to HPLC December 2016

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    The document is an introduction to high performance liquid chromatography (HPLC) presented by Robbie Coffin of Industrial Microbiological Services Limited (IMSL). It provides a brief history of HPLC, explaining it was developed in the early 20th century by Russian scientist Mikhail Tswett. The document defines HPLC and describes the basic components of an HPLC system. It also outlines the main modes of HPLC including normal phase, reverse phase, size exclusion, and ion exchange. Finally, it states that HPLC services are provided by IMSL.

    Droits d'auteur :

    © All Rights Reserved
    Téléchargez comme PPT, PDF, TXT ou lisez en ligne sur Scribd
    Signaler comme contenu inapproprié
    17 vues8 pages

    Introduction To HPLC December 2016

    Transféré par

    Robert Coffin
    The document is an introduction to high performance liquid chromatography (HPLC) presented by Robbie Coffin of Industrial Microbiological Services Limited (IMSL). It provides a brief history of HPLC, explaining it was developed in the early 20th century by Russian scientist Mikhail Tswett. The document defines HPLC and describes the basic components of an HPLC system. It also outlines the main modes of HPLC including normal phase, reverse phase, size exclusion, and ion exchange. Finally, it states that HPLC services are provided by IMSL.

    Droits d'auteur :

    © All Rights Reserved
    Téléchargez comme PPT, PDF, TXT ou lisez en ligne sur Scribd
    Signaler comme contenu inapproprié
    sur 8

    IMSL

    Tel + 44 (0) 1252 627676


    Fax + 44 (0) 1252 627678

    industrial microbiological services limited http://www.imsl-uk.com

    Introduction to HPLC

    Robbie Coffin
    IMSL, UK

    Introduction to HPLC by Robbie Coffin, IMSL IMSL, December 2016


    IMSL
    Tel + 44 (0) 1252 627676
    Fax + 44 (0) 1252 627678

    industrial microbiological services limited http://www.imsl-uk.com

    Overview
    • A Brief History
    • What is HPLC?
    • HPLC at IMSL

    Introduction to HPLC by Robbie Coffin, IMSL IMSL, December 2016


    IMSL
    Tel + 44 (0) 1252 627676
    Fax + 44 (0) 1252 627678

    industrial microbiological services limited http://www.imsl-uk.com

    A Brief History
    Mikhail Tswett (1872 – 1919), a
    Russian boanist, developed the
    chromatographic method.

    The chromatography comes from


    the greek“chroma” which means
    “colour”, and “graphein” which
    means “to write.”

    Introduction to HPLC by Robbie Coffin, IMSL IMSL, December 2016


    IMSL
    Tel + 44 (0) 1252 627676
    Fax + 44 (0) 1252 627678

    industrial microbiological services limited http://www.imsl-uk.com

    What is HPLC?

    Introduction to HPLC by Robbie Coffin, IMSL IMSL, December 2016


    IMSL
    Tel + 44 (0) 1252 627676
    Fax + 44 (0) 1252 627678

    industrial microbiological services limited http://www.imsl-uk.com

    Instruments

    • Solvent
    • Solvent Delivery System (Pump)
    • Injector
    • Sample
    • Column
    • Detectors
    • Waste Collector
    • Recorder (Data Collection)

    Introduction to HPLC by Robbie Coffin, IMSL IMSL, December 2016


    IMSL
    Tel + 44 (0) 1252 627676
    Fax + 44 (0) 1252 627678

    industrial microbiological services limited http://www.imsl-uk.com

    Modes of HPLC
    • Normal phase

    • Reverse phase

    • Size exclusion

    • Ion exchange

    Introduction to HPLC by Robbie Coffin, IMSL IMSL, December 2016


    IMSL
    Tel + 44 (0) 1252 627676
    Fax + 44 (0) 1252 627678

    industrial microbiological services limited http://www.imsl-uk.com

    HPLC at IMSL

    Introduction to HPLC by Robbie Coffin, IMSL IMSL, December 2016


    IMSL
    Tel + 44 (0) 1252 627676
    Fax + 44 (0) 1252 627678

    industrial microbiological services limited http://www.imsl-uk.com

    Thank you for listening!

    Any questions?

    Introduction to HPLC by Robbie Coffin, IMSL IMSL, December 2016

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