Silay is popularized for its pond that is

habituated by a lot of contaminated organisms

and poisonous chemicals due to people’s
unethical actions. Mudfish is one of the species
that is greatly affected by the contaminants.
Heavy metals cannot be simply terminated to
the point that they will be mixed up with water
along with the aquatic organisms specifically
Mudfish (Clarias anguillaris) to be discussed in
this study.
Heavy metals found in the study conducted by J.
Abah’, et al. (2013) were As, Mn, Cu, Pb and Fe.
Lead, copper, nickel and zinc are mostly found in
sediments. Water body pollution by heavy metal
in recent times has become a problem of
increasing public concern due to their gradual
increase in excess of natural background resulting
to deterioration in water quality for consumption
as well as threatens aquatic life including mudfish
and other fishes (O. Ebere’,et al., 2010).
Heavy metals may enter fish bodies in three
possible ways (via digestive tract, body surface
and gills) (Ushie O.A, 2010).
The study was made to establish the
following objectives:
 To identify the heavy metals in
Neochanna burrowsius.
 To figure out the things that the
environment of the organism,
Neochanna burrowsius, give to its body.
 Mudfish. It is used as a subject to collect the
analysis of the heavy metal.
 Fishing Rod. It is use to get the specimens.
 Water: sediment extraction. It is used for
analyzing the water in the location if it affected the
specimen.
 Laboratory analysis. It is use to determine the
distribution of heavy metal in specimen.
 Study Area. Silay pond, Surigao City Surigao Del
Norte this is where the specimen found/get from
its environment.
Samples Collection and Preservation
Samples of mudfish (Neochanna burrowsius), water
and sediment were collected from fishing locations
Silay pond. It lives and feeds from the bottom
sediment of the pond. The sediments were collected
by scooping with plastic hand trowel (0.25cm deep)
and immediately put in sterilized polythene bags,
closed and labeled. They were taken to laboratory
and dried at 40°C in an oven, prior to analysis
(Maitera et al., 2011).
Fish samples
The fishes were descaled and eviscerated and then
transferred into electric oven at 40oC. They were
dried in the oven at this temperature for 24hours and
then pulverized in a clean dry porcelain mortar
(Ahmed et al., 2010). The pulverized samples were
dried further for 1hour at a reduced temperature of
20oC and put into clean dried bottles (Farkas et al.,
2000; Adeniyi et al., 2005).
Analyses of the variations of concentrations of the heavy
metals were carried out using t-test and results were
considered statistically significant at p < 0.05. The analysis of
variance (ANOVA) was used for detection of differences in
metal concentrations between tissues significant difference
test was employed. Furthermore, the obtained data were
statistically analyzed by t-test for assessment of variation in
metal concentrations among small and large size individuals
within each species and also between two different species.
Pearson's correlation coefficients were used when calculating
correlations between fish of concentration of metals.
Moreover, bioaccumulation factor between fish and water was
calculated, using the mean metal concentrations in fish and
the corresponding metal concentrations for water.