Genetic Engineering

Steps
General Biology 2
A white crow? CROW
Have you heard from elders the lines about these crow?
Seriously? Now this crow is making history because elders always
say that they will grant the wish of the child only if ever a
crow becomes white.

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The steps in Genetic Engineering
STEP 1 STEP 2 STEP 3 STEP 4 STEP 5

Isolate plasmid Isolate DNA Cut the plasmid Cut gene of Combine the gene
interest and the plasmid

Isolate the plasmid from Isolate the DNA of human
bacterial chromosome cell that has the ability to
produce less melanin
Plasmid is being cut by a The human DNA is being This combining of the
restriction enzyme cut with the same enzyme plasmid and the gene of
specific to the restriction interest is possible with
site of the human gene. the help of the enzyme
ligase that serves as a glue
to stick the two together.

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NEXT STEP
STEP 6 STEP 7
Put recombinant Allow Bacterium to The protein is
DNA into bacterial reproduce isolated from the
cell cloned bacterium
This process of putting The bacterium carrying
back the plasmid into the recombinant DNA is
bacterial cell is what you being selected, allowing
called transformation them to multiply by
themselves.
STEP 8
Having many clones of
bacterial cell, we need to
isolate the protein
carrying the white trait
that we wanted to
express.
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 It’s now time to make
this crow white
The resulting gene that would express the white
feather trait is now inserted to the egg of the
crow enabling the crow to have a white feather.

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 Summary
With genetic engineering, we modify organisms
in a good way

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Thank You
MA. ANTHONEE LIANNE C.
CUADRO