Preparation of Media Culture

By:
Henni Floris Nahampun
Lestri Fanlina Sitohang
Naomi Lamtiur Napitupulu
Novita Manalu
 Outline
• Definition of culture media
• Good Media Requirements
• Materials for Media Preparation
• Kinds of culture media
• Procedures of media preparation
 Definition of Culture Media
• Medium is a substance consisting of a mixture
of food substances (nutrients) used for the
maintenance and growth of microorganisms.
 Media Requirements
• The medium should contain all the nutrients
that are easy to use by microbes
• The medium must have osmotic pressure;
• The medium contains no inhibiting substances;
• The medium should be sterile, no
contaminants from undesirable
microorganisms
Materials for Media Preparation
• Basic Materials
 Water as the solvent
 Agar for the media comfactor
 Gelatin also used as the comfactor but it can
be elaborated by microorganism
Gel Silica used as the media comfactor for the
obligate autotrof microorganism
Materials for Media Preparation
• Nutrients
Macro elements: Carbon, Hidrogen, Oxygen,
Nitrogen, Phospor
 Micro elements: Fe, Mg and trace element
Materials for Media Preparation
• Aditional Materials
Used for certain purpose
 example, phenol red used as the indicator of
pH changing as the result of organik
production during metabolism
 antibiotic used to inhibit the growth of non-
target microorganism/contaminants
 Kinds of Media
A. Based on Consistency
 Liquid media: media that is not added to the
compacting material
Semi-solid Media: containing agar for less
than 0.3% - 0.4% so that the media becomes
supple, not dense and not very liquid.
Solid Media: contains a lot of agar or a
compactor of about 15% so that the medium
becomes solid
 Kinds of Media
B. Based on Composition
 Natural / non-synthetic media is a medium composed
of natural ingredients in which the composition cannot
be known with certainty. For example Tomato juice
agar.
 Semi-synthesis media is a medium composed of
natural ingredients and synthesis materials. For
example, nutrient broth prepared from Pepton, Meat
Extract, NaCl and Aquadest.
 Synthesis media is the media composed of chemical
compounds where the type and dosage is known for
sure. For example Mac Conkey Agar.
 Kinds of Media
C. Based on the Function
 Basal media (basic media): a medium used as a base
material to make other media more complex. For
example, nutrient broth, pepton broth, etc.
 Selective media: enhance the growth of certain
organisms while inhibit others due to the inclusion of
particular substrate. For example Salmonella Shigella
Agar (SSA), Thiosulphate Citrate Bile Salt (TCBS), etc.
 Differential media: allow identification of
microorganisms usually through their unique. For
example, Triple Sugar Iron Agar (TSIA) Media, Indol
Motive Slimit Media (SIM), etc.
 Enriched media: contain specific growth factors that
allow the growth of metabolically fastidious
microorganisms. For example, Chocolate media and
Yeast-Extract-Potassium Nitrate Agar
 Enrichment media: contains certain materials where one
side can inhibit the growth of certain bacteria, but on the
other side can support the growth of certain other
bacteria. For example Muller-Kauffman media.
 Test media (identification) is a medium used for
microbial identification, generally coupled with certain
substances that become indicators. such as medium litmus
milk.
 General media: a media that is added ingredients that
aim to stimulate microbial growth in general. For
example, Nutrient Agar (NA), Potato Dextose Agar
(PDA).
 Spesific media: for determining the type of microbial
growth and its ability to make certain chemical changes.
for example medium tetes tebu for Saccharomyces
cerevisiae
• Testers media (Assay media): a media with a particular
arrangement used for testing certain compounds with the
help of bacteria such as medium to test vitamins,
antibiotics and others.
Media Preparation