EFFECTIVENESS OF

MAKAHIYA (MIMOSA PUDICA )

AS A PESTICIDE AGAINST
RICE BLACK BUG TURTLE
(AMAUROCHROUS DUBIUS)

Kirsten Cassandra P. Pascual

Jazreen Licelle M. Dorlones
Candace Nathalie N. Casia
 INTRODUCTION
Rice is a primary source of food for more than half
of the world population, and it provides income and
employment to more than 100 million households in Asia
and Africa. Approximately 52% of the global production of
rice is lost annually owing to the damage caused by biotic
factors. More than 100 species of insect pests attack the
rice crop including the Rice Black bug turtle. The species
complex is known to vary in abundance and distribution
from locality to locality and from year to year (Bhatt et al.
2018).
 In the northern part of India there has been observed
to consume large amounts of WHO classified “extremely” and
“highly hazardous” pesticides, in rice crop, posing significant
health and environmental threats. Custody in view this
problem, Integrated Pest Management (IPM) modules were
synthesized for rice crop and then compared with non-
IPM/farmer’s practice (NIPM).The IPM modules were observed
to have least impact on natural enemies with 30–40% increase
in population, while keeping the weed population below 10%.
NIPM, on the other hand, had resulted in 20% reduction in crop
yields, 50% reduction in biodiversity, and about 150% increase
in weed population, relative to the control (untreated) rice
fields (Arora et al. 2019).
 On the other hand, Davao City farmers
and technicians noted the presence of the Rice
Grain Bug (RGB), which at first glance appeared
to be a tiny species of praying mantis or black
ant in Maragusan, Compostela Valley. Rice
farmers in Negros Occidental suffered losses
amounting to PHP11.544 million brought by
various crop pests in the past four months
(Provido, 2017).
 Makahiya plant extract has strong
antimicrobial activity against Staphylocus
aurens and Bacillus Subtilis. Makahiya extract
contains the phytochemicals alkaloids,
flavonoids, saponins and triterpenes. Makahiya
plant extract has strong anti-microbial activity
against Staphylococcus aureus and Bacillus
subtills but has negative activity against Candida
albicans and Makahiya ectract exhibits
antioxidant property.
 This study can contribute towards
unraveling new knowledge and researches in
understanding the effects of Rice black Bug
Turtle to the rice fields that leads to rice
shortage control through the Makahiya Extract
that can be seen anywhere .
MATERIALS AND METHOD

This study is composed of three phases,

Phase 1 Gathering of Materials and preparation,
Phase 2 Makahiya extract experimentation,
Phase 3 data Analysis. All experimental
procedures where done in the science
laboratory at Cabantian National High School.
 P H A S E 1 : G AT H E R I N G O F
M AT E R I A L S A N D P R E PA R AT I O N

A. Collection of Makahiya

The Makahiya (Mimosa Pudica)Plant was

collected from the backyard of the
proponents.
B. Collection of Rice Black Bug Turtle

Rice Black Bug Turtle was collected using

the Sweep net sampling at the
Banaybanay farm of the proponents.
PAHSE 2: MAKAHIYA EXTRACT
AND RICE BLACK BUG TURTLE
EXPERIMENTATION
A. Collection of Makahiya Extract Samples
The first step is to wash the Makahiya plant , cut the stem and
the leaves into smaller pieces. Next, separate the Makahiya Leaves into
the Makahiya stem. After that, pound the stems with the mortar and
pestle to get the extract. Then, put the leaves and the stem into a pot and
let it boil after 5 minutes. Put the Extract to the container with
cheesecloth and let it cool for several minutes. 145 ml of extract was
collected in the experiment.
B. Makahiya and rice Black Bug Turtle set-up

The experiment was composed of four sets(Set-A 2 rice

black bug and Spray of Makahiya extract, Set-B 2 rice black bug and
2 Sprays of Makahiya Extact, Set-C 2 Rice Black bug and 3 sprays of
makahiya Extract, Set-D 2 rice Black Bug and 4 Sprays of Makahiya
extract ). 8 Rice Black Bug Turtle and 145 ml of Makahiya Extract.
C. Experimentation, observation and Evaluation

After the expermientation, it is observed that in the first set

no significant changes was observed . In the second set, 1 rice black
bug died in 5 minutes. In the third set, both rice blacg bug died in 5
minutes. But in the last set, both died in 2 minutes.
D. Disposal of Rice Black Bug Turtle samples

The dead Rice Black Bug Turtles were

burried in the backyard of the proponent’s
house.
PHASE 3: DATA AND
ANALY SIS

After gathering results, it is observed

that the more sprays of makahiya extract,
the more Rice Black bug Turtle died in a
short period of time.