BCC-3000B Instrument

Service Manual
----Changchun Dirui Industrial Co.,Ltd.
 BCC-3000B
Automatic Hematology Analyzer

Hematoloty Analyzer

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 Principle
This hematology analyzer adopts electrical impedance
method to test amount of red blood cell.white blood cell and
platelet as well as volume distribution. Test hemoglobin
concentration by colorimetric method.and based on this.to
calculate other parameters.
Electrical impedance illustration：

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 Characteristics
● Accurate and reliable result
Using digital filtering.Blood cells overlap calibration.fitting curve.Floating
landmark technology.etc. Ensure accurate and reliable result.
● Perfect quality control function
L-J, X, X-B three types of quality control mode.
● Easy maintenance
1.Hypertension calcined and dual way high pressure cleaning.effectively
avoid aperture blocking
2.Electro hydraulic separation structure design
3.Automatic sensing the remained reagents.reminding user to change
reagent in time
4.Specific boot program.it can clean and maintain the liquid tube system
automatically.which keeps the clean tube system.
● large storage
Large storage memory can store up to 30000 testing results and could be
extended according to customers’ requirement.
● Cyanide-free environmental Reagent
Using cyanide-free environmental hemolytic reagent to test HGB.it is safe
and environmental.
● Easy operation
Using touch-screen LCD displays all parameters and histograms. It is
automatic sample loading.mixing and testing.

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 Technical Specification
• Sample Volume : Prediluted 20ul Whole Blood 13ul
• Aperture Diameter : WBC: 100um RBC: 70um
• Hole Depth : WBC: 70 um RBC: 65um
• Wavelength : 540 nm
• Test speed : 60 samples/hour
• Dilution rations : whole blood: WBC/HGB 1:308 、RBC/PLT 1:44,872
• Capillary Blood: WBC/HGB 1:428 、RBC/PLT 1:43,355
• Lyse Volume Whole Blood Capillary Blood
• 0.5 ml 0.36 ml
• Parameters
• White Blood Cell or leukocyte WBC
• Lymphocyte Lymph#
• Mid-sized cel Mid#
• Granulocyte Gran#
• Lymphocyte percentage Lymph%

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• Mid-sized cell percentage Mid%
• Granulocyte percentage Gran%
• Red Blood Cell or erythrocyte RBC
• Hemoglobin Concentration HGB
• Mean Corpuscular (erythrocyte) Volume MCV
• Mean Cell (erythrocyte) Hemoglobin MCH
• Mean Cell (erythrocyte) Hemoglobin Concentration MCHC
• Red Blood Cell (erythrocyte) Distribution Width Coefficient of Variatio RDW-CV
• Red Blood Cell (erythrocyte) Distribution Width Standard Deviation RDW-SD
• Hematocrit HCT
• Platelet PLT
• Mean Platelet Volume MPV
• Platelet Distribution Width PDW
• Plateletcrit PCT
• Platelet Larger cell ratio P-LCR
• WBC Histogram
• RBC Histogram
• PLT Histogram

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• Storage: 1000 testing results, 570 QC results
• Language: English
• Operation Environment:
• Temperature ：15℃～35℃
• Humidity ： 30％～85％
• Air Pressure ： 76kPa～106kPa
• Input/Output: RS-232. Internal thermal printer. 1 bar code
reader (optional).1 parallel printer(optional)
• Data transmitting rate: 19200bps
• Power supply: ~220V±22V 50Hz ±1Hz
• Fuse: 250V 2A
• Power: 200W
• Weight: 25Kgs
• Dimension: 460(L)*390(W)*410(H)
• Electromagnetic Compatibility (EC)
• Accord with EMC test standard EN61326：1997+A1
1998+A2 2001+A3 2003；
• Sound pressure : Max. 85dB

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Instrument structure
•Front view

3
2 4
5

1 -Screen 2- Printer 3 –Sample probe 4 –Counting button

5 –Bioharzad symbol

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 • Back view

9
1
10

2
4
3
8
5
7
6

1-External connection port（PRINTER） 2- Power switch

3-power socket 4-Dilution connection port
5-Cleaning liquid connection port 6- Waste connection port
7-Cleaning liquid sensor socket 8-dilution sensor socket
9- Communication port （ RS-232） 10-barcode reader port

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 Front view with door open
1

4 3

1-Elevation motor 2-Probe 3-Swab

4-WBC cover 5-RBC cover 6-Counter 5

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 View of left door open

2
3

1-Pressure bottle 2-negative pressure Pump

3-positive pressure pump

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View of right door open
1-valve8 2-volume counting unit
3-vaccum bottle 4-valve15 5-valve16
6-valve14 7-valve13 8-valve10 9-valve2
10-valve9 11-Motor with 50ul and 2.5ml
12-10ml motor 13-2.5ml syringe
14-50ul syringe 15-10ml syringe
16-valve4 17-valve3 18-valve1
19-valve6 20-valve5 21-valve17
22-valve7 23-valve18

Clinic Operation of Whole Blood Predilute

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 Parameter Introduction

• BCC-3000B can quantitively measure 20 items

parameter in blood plus 3 histogram

White Blood Cell or leukocyte

WBCLymphocyte Lymph#
Mid-sized cel Mid#
Granulocyte Gran#
Lymphocyte percentage Lymph%
Mid-sized cell percentage Mid%
Granulocyte percentage Gran%
Red Blood Cell or erythrocyte RBC
Hemoglobin Concentration HGB

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Mean Corpuscular (erythrocyte) Volume MCV
Mean Cell (erythrocyte) Hemoglobin MCH
Mean Cell (erythrocyte) Hemoglobin
Concentration MCHC
Red Blood Cell (erythrocyte) Distribution
Width Coefficient of Variatio RDW-CV
Red Blood Cell (erythrocyte) Distribution
Width Standard Deviation RDW-SD
Hematocrit HCT
Platelet PLT
Mean Platelet Volume MPV
Platelet Distribution Width PDW
Plateletcrit PCT
Platelet Larger cell ratio P-LCR

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Parameters from instrument measuring

• White Blood Cell or leukocyte

WBC
• Red Blood Cell or erythrocyte
RBC
• Hemoglobin HGB
• Platelet PLT

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 Parameters from Histogram
Name ab. Default unit
Lymphocyte percentage Lymph ％
Mid-sized cell percentage Mid ％
Mid-sized cell percentage Gran ％
Mean corpuscular (erythrocyte)volume
MCV fL
Red blood cell(erythrocyte) distribution width coefficient of
variation percentage RDW-CV ％
Red blood cell( erythrocyte) distribution width standard
deviation RDW-SD fL
Mean platelet volume MPV fL
Platelet distribution width PDW ／

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 Calculated Parameter

Name Ab. Default Unit

Lymphocyte Lymph# 109 ／L
Mid-sized cell Mid# 109／L
Granulocyte Gran# 109／L
Red cell blood HCT ％
Mean cell( erythrocyte) hemoglobin
MCH pg
Mean cell (erythrocyte) hemoglobin concentration
MCH Cg／L
Plateletcrit PCT ％

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Operation procesure of daily measurement
Preparation operation

Start up

Daily control

Sample preparation

N
Whole blood Prediluted sample analysis

Y
Whole blood sample analysis

Shut down

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Preparation of operation

Before switching on the analyzer power.the operator should

inspect as follows to make sure the system is ready.
1． Inspect liquid waste barrel
The operator should prepare liquid waste barrel by himself.and
make sure that is empty before starting up.
2． Inspect liquid pipeline and power.
Inspect the pipelines of diluent.cleaning solution and liquid
waste.check if they have kinked or connected well.
Inspect analyzer power plug is inserted into the socket.
3． Inspect internal thermal printer.bar code reader, external
printer.
Inspect if there have enough paper in printer and fixed right.
Inspect if power supply of external printer and barcode reader
have inserted into the power supply socket and cable had been
connected with the instrument .

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Before analysing sample.the operator should perform the analysis
of quality control of analyzer everyday to make sure the results of
analyser are credible.
Preparation of sample
whole blood sample
Preparing method of WB sample as follows
1．Use K2EDTA(1.5—2.2mg／mL blood)anticoagulant vacuum
valve to gather venous blood sample.
2．Mix the venous blood and anticoagulation in vacuum valve
rapidly and adequately. Notice
● Operator should use clean K2EDTA anticoagulant vacuum valve,
silicified glass/ plastic test tube and 20uL
● The samples that need to be done leucocyte 3-part differential
and haematoblast analysis should bekiip at normal ambient
temperature.and they should be analyzed within 8 hours after
they’re gathered.
● If operator does not need the results of PLT. MCV and leucocyte3-
part differentia.the samples can be kept for 24 hours in fridge at 2℃
一8℃. If the samples are refrigerated.they can be used to analyse
after 30 minutes.
● The sample placed for a period of time should be used after
being remixed.
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Lift up switch of front door locker according to the arrow direction.
and open the front door.

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Use clamp or forfex to cut off the line-packing.and let the sampling
probe get back to the normal condition.

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 Sampling probe after taking off line-
packing

Pull up the locker switch.close the front door.press down the

locker to lock the front door.and close the right door

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 Reagent connection
Note:
● Please do not leave the reagent above the analyzer.
● Must use the manufacturer matched reagents.
● Keep the reagent static for a while and keep waiting until it
is stable before using.
● Make sure to use the reagent within the period of validity in
the reagent instruction.
● Cover the reagent bottle lid after reagent connected to the
analyzer in case of the reagent pollution.
As for the finished analyzer. install plastic protection lid at every
liquid circuit of analyzer in case of liquid circuit system pollution
during trans. Take off the protection lid and preserve properly
before reagent connected.

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 Hemolytic agent connection
Pull the left door locker according to the arrow direction to open
the left door

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2. After opening left door.the joints of black sensor and white liquid path
shown in the picture.

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3. Take out cover groupware of hemolytic agent from accessories box.
Open the cover of hemolytic agent . put one side which is direction of
transducer side into hemolytic agent . Follow the clockwise close the cover

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4. Put the bottle of hemolytic agent on the left side of bracket.
according to pipeline guide.make connect with joints of transducer
and liquid circuit.

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 Diluent connection

1. Take out cover groupware of diluent form

accessories box.
2. Rive off the hole which is on the top of diluent
picking box.have bottle cover inside.put picking
box to the operate station as same level as
analyzer.
3. Open the bottle of diluent.put one side which
is direction of sensor side into hemolytic agent.
Follow the clockwise close the cover
4.According to the mark and make connect
between diluent pipeline with analyzer

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Insert BNC plug connecting with lead to
diluent sensor BNC socket in the back of
instrument and screw it tightly.

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 Cleaning liquid connection

1．Take out cover groupware of cleaning liquid from

accessories box
2．Rive off the hole which is on the top of cleaning
liquid picking box.have bottle cover inside.put
picking box to the operate station as same high level
as analyzer.
3．Open the bottle of cleaning liquid.put one side
which is direction of transducer side into hemolytic
agent . Follow the clockwise close the cover
4. According to the notice and make connect between
diluent pipeline with analyzer.Follow the clockwise
close the cover
5. Plug the wire plugs to the cleaning liquid sensor
socket.

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 Waste liquid pipeline connection

1. Take out depose liquid pipeline from the

accessories box.
2. According to the mark which is on the
pipeline.and make connection between liquid
pipeline with calculater instrument..
3. Put the depose liquid bottle under the
operate station.
4. Put another side of pipeline for depose
liquid into the depose liquid bottle.

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 Printer paper installation

1.Press upper -middle of printer cover.open the cover.

the figure as follows

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2. Lift up the printer pressing-bar.rive off the protect velum of
printer paper.and leave the print side facing down.plug triangle
head under printer roller.and push by hands. Feed the paper in
the printer groove.as shown in figure.

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3. Press the printer thermistor pressing-bar.as shown in figure。

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 4.Close printer cover.as shown in figure。

notice
● Do not insert or dial printer or bar coder reader when instrument performs
opening processing.
● Must be using the external equipment by specified type.

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 Connect with stylus printer
the instrument can connect with stylus printer.
through external stylus printer get output
measurement result.connect one and of printer wire
to the printer.the another end to the serial port of the
outer printer outside the instrument.

Notice
BCC-3000B automatic hematology analyzer can connect with
external printer by EPSON LQ 1600K types.and EPSON
LQ300K stylus printer

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 Connect with bar code reader
BCC-3000B automatic hematology analyzer can
connect with bar code reader and receive the bar
code scanned by bar code reader.the method to
connect as figure 1-19. insert the bar code reader
into the port at the back case of analyzer.
The barcode reader which belongs to the
customer spare part.if the customer purchases our
handheld barcode reader.only need to insert the
connector of bar code reader into the port of BCC-
3000B hematology analyzer. The communication
agreement have already set up before shipment. The
customer can directly use.

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 Connect with computer
The instrument can connect with computer by the cable.transfer
the data to computer.insert one end of the communicate cable
into the serial port (figure1-9) and the other end into the serial
port of the computer.

Caution

Switch of power supply must be closed before installing power wire:

· End of earthing wire contacts earth well
· Connect the equipment possibly producing interfere with other
plugs.
· Forbid connecting earthing wire to pipeline.such as:
air pipeline.

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 Reagent introduction

Hemolytic agent：
Hemolytic agent is used to test amount of leucocyte and hemoglobin
concentration in whole blood by in-vitro quantitive measurement.
Applied range：available to CA-500 & BCC-3000B etc.hematology
analyzer.

Note:
1、During hemolytic agent preservation, be sure not to
freeze.
2、Storing temperature is 2~30℃ .
3、The period of validity of unopened product is one
year. The period of stabalization of opened prouduct is 90 days.
4、Make sure not to interfuse impurity during using.
5、Don’t touch skin directly.
6、Keeping away from light to preserve
7、The tested sample should be fresh blood.

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Dilute Blood：
Usage：Dilute blood can completely keep corpuscle’s
original volume in one period of time.
Applied range：available to CA-500 & BCC-3000B
etc.hematology
Using method：Reference to user manual

Note:
1 During hemolytic agent preservation.be sure not to freeze.
2、Storing temperature is 2~30℃ ；
3、The period of validity of unopened product is one
year. The period of stabalization of opened prouduct is 90 days.
4、Make sure not to interfuse impurity during using

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Mechanism structure.
Disassembly.
Maintenance.

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 Installation notice
1. Installing pressure pump pays attention to the positive and negative pole.
2. Notice if there are substance covering the joint seam

Pressure pump

Negative pressure pump

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 BCC-3000B Plate Module

• CPU Main control plate

• Power driver plate
• Simulating plate
• Volume meter plate
• Printer driver plate

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 CPU Main Control Plate
• It is composed of several parts: Computer system. Display module
circuit. A/D module circuit.I/O module circuit.

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Power driver plate
• Accroding to the order of CPU.power driver plate carries out the
operation to rotatory motor. fluctuating motor and probe
motor.and to switch controlling pump and valve. It has three
main modules: switch.motor and power control module.

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 Simulating plate
• Simulating plate mainly transforms the original signal to the one meeting A/D
requirement. It includes : WBC and RBC+PLT magnification unit.HGB magnification unit ,
temperature measuring unit.vacuum measuring unit . Power monitor unit ( change all
output value into 0 to 5v voltage available for A/D conversion) .and plus power supply
unit.pore voltage disposal unit. electronic simulating switch etc.

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 Volume meter plate
• Volume meter plate guarantees the precision of WBC and
RBC+RLT by burette and measure photocoupler calculating liquid
volume

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 Printer driver plate
• There is a SMC on the printer driver plate.which communicates with
CPU by serial port and main control plate.print out the character and
wave shape according to the CPU order.and send the status of
recorder to main CPU for display.

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Liquid circuit and its sequence

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Valve : Magnetic exchange valve.6 three channels,
12 two channels.
Pump: one liquid pump.one pneumetic pump.
Syringe: one per 2.5ml .10ml. 50ul.
Sampling probe : one
Rinse mop: one
Vacuum room: one
Pressure room: one
WBC Counting cell: one
RBC Counting cell: one

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Troubleshooting

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 Malfunction and resolvent
If analyzer detects some unnormal situations, the relevant malfunction
reminding will show on the screen. The following is the guidelines for
resolving problem.

WBC original value high

1．Check if there has earthing wire inside of the instrument .
2．Inspect the histogram on the screen.
3．Immerse the counting cell with probe washing solution .
4．Open the front door to avoid electric circuit interfere.
5．Observe if there are bubbles in 10ml syringe.
6．Detach,wash the front and rear side of the counting cell and the aperture.
Replace the gasket of aperture
7．Stop adding hemolytic agent to avoid the interference .
8．Observe the connection tubes under the counting cell.clean the
underside pipeline if it is dirty
9．Valve 12 is dirty. Unable to close tightly and open smoothly 10．Replace
counting cell .
11．Fix the Simulating plate if it ‘s broken .

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 PLT Original value high

1． If the original value are stable.try to replace reagent .

2．Check if there has earthing wire .
3．Observe the histogram .
4．Immerse the counting cell with probe washing solution .
5．Detach.wash the front and rear side of the counting
cell.and the aperture. Replace the gasket of aperture
6． The PTFE tube under the counting cell is dirty .
7． Unable to empty the counting cell. V11 Valve dirty.
Unable to close tightly and open smoothly .
8． Replace the counting cell.
9. Aperture
10．Simulating plate

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 WBC Blood sample value high

1．Check if the original value is normal .

2．Check if the demarcate coefficient is correct .
3．Whether there has lyse,.check the adding volume .
Examine the 2.5ml syringe and valve 6 .
4．Valve 12 is dirty.valve plate’s broken .
5．Simulating plate

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 HGB Adustment Warning
• Look over the original voltage of HGB

• If it is stable.but low:
• 1. Immerse rinse the counting cell of WBC .
• 2. Adjust the original voltage of HGB.adjust VR3 of simulating plate .

• If it is unstable
• 1. There are dirty stuff in the counting cell of WBC. rinse the cell .
• 2. Whether there has water film between the lateral cell and the HGB
rack .
• 3. The line connecting of sending and receiving of HGB. replace HGB
unit .
 RBC.RLT Blood Sample Value Low
1．Unable to empty the counting cell .unable to open /close
valve smoothly
2．Wether sampling probe performs adding and diluting
sample normally .
3．Simulating plate

WBC Blood Sample Value Low

1．Unable to empty the counting cell .unable to open /close
valve 12 smoothly. ( RBC value is also low at that time)
2．Sampling probe’s blocked.sampling volume is not
enough. (RBC value is also low at the time)
3．There is leakage between sampling probe and 10ml
syringe . (RBC value is also low at the time)

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 WBC/RBC Aperture jam
1．Check counting time
2．Check aperture voltage
3．Whether mistake the diluent by cleaning liquid.whether
reversely connect the dilutent and cleaning liquid. .
4．Press dredging key. incandesce. backflushing
5．Check whether have Backflushing.
If not. Immerse aperture by washing liquid for 20 minutes
Check V8 V18 V9
If does. Observe the statement of flowmeter
Check V8/V18

Press rinse key in the display . there don't have washing liquid
in one side or both sides of flowmeter.
（1）．No reagent
（2）．Outer pipeline jam ( pressed)
（3）．Inner pipeline jam ( fold )
（4）．Cell or pipeline is leakage
（5）．V13/V14 malfunction

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 Air bubble in WBC/RBC
Simultaneously appear air bubble at both sides
1．If there have diluent
2．Observe whether have diluent in both cells.
3．Observe whether 10ml syringe is full or not. If not. syringe or
its pipeline has leaked .
4．The upper pipeline of 10ml syringe fold. Which caused jam .
( 10ml motor malfunctional alarm )

One side of air bubble

1、Rear cell is leakage .
2、V8/V18 is broken .
3、Pipeline leaks .
4、V7/V17 cannt be closed tightly .

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Blood sample counting: WBC value is normal.RBC
value is less than 1. PLT value is zero or several tens or
normal

1．Diluent and washing liquid connect reversely .

2．Vacuum value is low.
3．Waste tube is pressed.discharging liquid not smoothly.
4．Vacuum filter is blocked .
5．The malfunction of vacuum pump .
6．Valve is not closed tightly.
7．Vacuum tank and its relevant pipeline leak .
8．The pipeline of vacuum sensor on simulation plate had
blocked by crystal .

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 WBC can not classify or not well.

1．Check amount of WBC .

2．Check the original value.eliminate the interfering factor .
3．Check if histograms are reduced .
4．Check aperture voltage .
5．Whether diluent is matching of analyzer and has expired or
not .
6．Whole blood or capillary blood.if anticoagulant is EDTA K2 .
check its proportion .
7．Check the status of sample and its standing time .
8．Check the result of temperature test .
9．Whether WBC gain is too little .
10．Check simultating plate .

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Fluctuating motor/ rotatory motor malfunction

1．Temperature is too low .

2．Motor is too large .
3．Upper photocoupler is broken .
4．Lower deflection photocoupler broken .
5．Motor is broken .
6．Power driver plate is broken .

There is no response and nothing shows on .

screen when turning on instrument .
1．If indicator light of power is on and its statement .
2．If there shows “initializing”.
3．Electronic plate is broken .
4．Main control plate is broken .
5．Power driver plate is broken .

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 Thank you for your long-term
support to Dirui! Tomorrow's Dirui will
provide more qualitied products and
services to our customer.and
contribute to the cause of human
healthy.

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