Organizing Science

Research Papers (11)

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Proline-rich tyrosine kinase 2PYK2, a non-
receptor phosphorylation kinase, belongs to the focal
adhesion kinaseFAKfamily.
Although in vitro experiments have indicated that
PYK2 is associated with macrophage mobility, adhesion
and regulation, such an association has not been made
through in vivo experiments.
For instance, PYK2 is deleted in the cell
as a mouse macrophage can affect the phagcytosis
function.
The inability to observe its association
through in vivo experiments makes its impossible to
understand how the role of PYK2 in macrophage in vivo.
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Based on the above, we should develop a novel animal
model that can be observed through macrophage in vivo experiments.
To do so, the regulatory role of a gene in the upstream
as promoter and enhancer can be analyzed by adopting molecular
cloning methods. Next, the identified gene can be linked with a
regulating gene to clarify how gene expression sites are regulated.
As anticipated, the proposed animal model can observe
macrophage mobility and life cycle, thus facilitating embryo
development research.
Importantly, the proposed model is highly promising for
use in related bio-medicine fields.
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Gene expression requires that many proteins interact
with regulated elements. However, modulation of gene
expression has not been thoroughly investigated, making it
impossible to determine the period and location of gene
expression.
However, expression of the same gene in a diverse
cell can be regulated based on different expressions and
, thus, cannot be expressed 100%.
The inability to thoroughly understand the
mechanism of distinct gene expression makes it impossible to
understand how this unique mechanism affects embryo
development.
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Based on the above, we should elucidate the mechanism of
gene expression in a cell owing to the importance of acquiring genes in
a cell for embryo development.
To do so, the regulatory role of a gene in the upstream
as promoter and enhancer can be analyzed by adopting molecular
cloning methods. Next, the identified gene can be linked with a
regulating gene to clarify how gene expression sites are regulated.
As anticipated, analysis results can clarify the mechanism
of gene expression in cells and regulation of tissue-specificity genes.
Results of this study can provide a valuable reference for
efforts to develop a therapeutic method of gene expression for patients
with genetic defects.
Further details can be found at
http://www.chineseowl.idv.tw