Oviposition deterrent and Ovicidal

effects of the leaf extracts of Parthenium hysterophorus

against an indian strain of Aedes aegypti

ANUBHA SETH
ABHAY PRATAP SINGH
Department of Zoology , Acharya Narendra Dev
College University of New Delhi 110019
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 Parthenium ~ at a glance!!
 Parthenium popularly known as
Congress weed.

 Accidentally introduced in India in

around 1956 and has since spread
over most part of the country.

 Contact causes dermatitis in human

and respiratory problems.

 Parthenium is reported as promising

remedy against ~ Hepatic amoebiasis,
Neuralgia.

 Jamaica: Used as a Flea-repellent both

for dogs and other animals. 2
 Larvae ~ collected from Delhi

Colony maintained~ 28 + 1oC, 80 + 5% RH

Adult food ~ Unseeded raisins

Blood meals ~ female mosquitoes ~ restrained albino rats in the cages

Eggs collected in bowls

Kept in cages and allowed to hatch in enamel trays

Larvae were fed upon a mixture of yeast powder ,grinded dog biscuits .
.
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  The powdered leaves was soaked
in 1000ml of:-
 Acetone, benzene, hexane ,diethyl
ether and petroleum ether
separately.

Now ,the soaked material

was left undisturbed for 5
days .
4
 Vaccum Rotary Evaporator:
 Use: To Concentrate the
crude extract.
 Concentrated at 45°C under
low pressure.
 Resultant

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 BIO - ASSAY

ER% = (NC – NT) X 100 ER-effective repellency

NC NC-no.of eggs in control
NT-No.of eggs leaf extract6
Solvent No.of Total no. N o. of Relative Total Hatch Per %
blood-fed of eggs Eggs Laid fecundity Hatch Female Hatch
females laid per (%
Female Repellency)

Control 13(39) 503 12.90 - 461 11.82 91.6

Petroleu- 13(39) 34 0.87 0.06 (93.2%) 20 0.51 58.6

m ether

Hexane 13(39) 131 3.32 0.25 (73.9%) 120 3.08 92.8

Diethyl 13(39) 02 0.05 0.003 0 0 0

ether (99.6%)

Benzene 13(39) 31 0.84 0.07 0 0 0

(93.8%)

Acetone 13(39) 149 3.82 0.30 139 3.56 93.27

 Parthenium

RESULT
OVI POSITION DETERRENT
 Diethyl ether 99.7% Max. repellency
Acetone 70-74% Least repellency
Hexane 70-74% Least repellency
OVICIDAL EFFECT
 Diethyl ether 100% Ovicidal effect
 Benzene 100% Ovicidal effect
 Petroleum ether 41% Ovicidal effect
Hexane and acetone 8-9% Ovicidal effect

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 Contd….
 Levels of active constituents in extract

 Responsible for the variability in their action against A.

aegypti.

 This Knowledge helps in designing and implementing an

effective strategy from a resistance-management
perspective against A. aegypti

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FUTURE PROSPECTIVE
 Botaincal extract can be used as an insecticides

 Isolating main Alkaloids to use in pure form

Major use : - Parthenium is used here as a bio agent to

control mosquito that is key factor specially in
Delhi,India.

 Making Parthenium more useable to control it

To study its effect on non-target organism 10