Science Project

Recommendations (11)


http://www.chineseowl.idv.tw


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(:)
In our working group meeting last week,
we discussed our laboratorys efforts to more fully
understand the role of proline-rich tyrosine kinase 2 (PYK2)
macrophage in vivo. PYK2, a non-receptor
phosphorylation kinase, belongs to the focal adhesion
kinaseFAKfamily. Although in vitro
experiments have indicated that PYK2 is associated with
macrophage mobility, adhesion and regulation, such an
association has not been made through in vivo experiments.
(NOTE : Add 2-4 sentences that describe characteristics of
the problem or statistics that reflect its severity)
For instance, PYK2 is deleted in the cell as a
mouse macrophage can affect the phagcytosis function.
The inability to observe its
association through in vivo experiments makes its
impossible to understand how the role of PYK2 in
macrophage in vivo.
(:)
Therefore, we recommend developing a novel
animal model that can be observed through macrophage in
vivo experiments. To do so, the regulatory
role of a gene in the upstream as promoter and enhancer
can be analyzed by adopting molecular cloning methods.
The identified gene can then be linked with a regulating gene
to clarify how gene expression sites are regulated. (NOTE:
Add 2 sentences to describe the methodology more
thoroughly) As anticipated, the proposed
animal model can observe macrophage mobility and life
cycle, thus facilitating embryo development research.
Importantly, the proposed model is highly promising
for use in related bio-medicine fields. (NOTE : Add 2-4
more sentences that describe more thoroughly how the
proposed method contributes to a particular field or sector)
(:)
Senior scientists have recently
expressed concern over the role of gene expression in
embryo development. Gene expression
requires that many proteins interact with regulated
elements. However, modulation of gene expression has not
been thoroughly investigated, making it impossible to
determine the period and location of gene expression.
However, expression of the same gene in a
diverse cell can be regulated based on different
expressions and (NOTE : Add 2-4 sentences that
describe characteristics of the problem or statistics that
reflect its severity) , thus, cannot be
expressed 100%. The inability to
thoroughly understand the mechanism of distinct gene
expression makes it impossible to understand how this
unique mechanism affects embryo development.
(:)
Therefore, we recommend elucidating the
mechanism of gene expression in a cell owing to the
importance of acquiring genes in a cell for embryo
development. To do so, the regulatory role
of a gene in the upstream as promoter and enhancer can be
analyzed by adopting molecular cloning methods. Next, the
identified gene can be linked with a regulating gene to clarify
how gene expression sites are regulated. As
anticipated, analysis results can clarify the mechanism of
gene expression in cells and regulation of tissue-specificity
genes. Results of this study can provide a
valuable reference for efforts to develop a therapeutic
method of gene expression for patients with genetic defects.
(NOTE : Add 2-4 more sentences that describe more
thoroughly how the results contributes to a particular field or
sector)
Further details can be found at
http://www.chineseowl.idv.tw