Writing Problem and Hypothesis

Statements for Science Research(2)

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Setting of work proposal :
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Work problem :
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Quantitative specification of problem :

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Importance of problem :
,
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Project need : ,
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Work objective : ?
Methodology to achieve objective
: ?
Anticipated results :
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Contribution to field :
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()
Acuolvirus expression system has been extensively
adopted in protein expression in recent years. However,
the conventionally adopted procaryote expression system fails to
compare posttranslational modification with the baculovirus expression
system. (NOTE : Add 2-4 sentences that describe characteristics of
the problem or statistics that reflect its severity)
Posttranslational modification of the baculovirus expression system is
better than that of the procaryote expression system. While producing
proteins such as those found in humans, this baculovirus expression
system requires less time than the mammalian expression system does.
The inability to use the baculovirus expression
system to produce proteins requires use of the mammalian expression
system to produce human proteins, which is inefficient and expensive.
Therefore, based on use of the baculovirus expression
system, an attempt must be made to achieve the expression of the
trypsin inhibitor, subsequently allowing this protein to inhibit cancer.
()
By using the baculovirus expression system, an attempt
can be made to achieve expression of the trypsin inhibitor,
subsequently allowing this protein to inhibit cancer.Posttranslational
modification of the baculovirus expression system is better than that of
the procaryote expression system, especially in terms of the protein
quality because it closely resembles that of humans.
To do so, a trypsin inhibitor can be produced using a baculovirus
expression system. Effectiveness of the trypsin inhibitor can then be
confirmed by western blotting. Next, a cancer cell can be terminated via
the trypsin inhibitor. As anticipated, the baculovirus
expression system can be used as an expression trypsin inhibitor to
promote anticancer activities. Results of this study can
provide valuable insight into how the baculovirus expression system
can be used to achieve the expression protein, given that this protein
closely resembles that found in humans. The baculovirus expression
system more closely resembles a human protein than the procaryote
expression system does. In practice, although this expression may not
be better than mammalian expression, the baculavirus expression
system requires a shorter time.
()
By using the baculovirus expression system, an attempt
can be made to achieve expression of the trypsin inhibitor,
subsequently allowing this protein to inhibit cancer.Posttranslational
modification of the baculovirus expression system is better than that of
the procaryote expression system, especially in terms of the protein
quality because it closely resembles that of humans.
To do so, a trypsin inhibitor can be produced using a baculovirus
expression system. Effectiveness of the trypsin inhibitor can then be
confirmed by western blotting. Next, a cancer cell can be terminated via
the trypsin inhibitor. As anticipated, the baculovirus
expression system can be used as an expression trypsin inhibitor to
promote anticancer activities. Results of this study can
provide valuable insight into how the baculovirus expression system
can be used to achieve the expression protein, given that this protein
closely resembles that found in humans. The baculovirus expression
system more closely resembles a human protein than the procaryote
expression system does. In practice, although this expression may not
be better than mammalian expression, the baculavirus expression
system requires a shorter time.
()
A syphilis diagnostic kit can be developed, capable of detecting
in only one step the anti-treponema specific antibodies in a patients serum or
plasma binding to the antigen. To do so, the serum samples
of syphilis patients can be analyzed. An experiment involving the syphilis
diagnostic kit can then be performed with the Treponema pallidum antigen,
TpN 15, TpN17 and TpN 47. Next, the gene recombinant method can be
adopted to produce traponemal antigens. Additionally, the fusion protein can
be used to achiee protein expression in the E.coli expression system.
Moreover, a syphilis recombinant system can be established usingrecombinant
technology. As anticipated, analysis results can indicate that a
fusion protein can be formed using TpN 15, TpN 17 and TpN 47. That protein
can then be used by gene recombinant technology to achieve procreation on a
large scale. Hopefully, product procreation on a large scale can elevate the
sensitivity of detecting syphilis disease to within an accuracy of 100%.
With a high degree of accuracy and specificity, the proposed syphilis
diagnostic kit can be applied for use in genetic engineering, protein
construction and reagent key technology. Moreover, the diagnostic kit is highly
promising for commercialization if the reagent can be developed successfully,
thus reducing the diagnostic time while increasing its accuracy significantly.
(NOTE : Add 2 sentences that describe more thoroughly how the proposed
method contributes to a particular field or sector)
Further details can be found at
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