Writing Science Abstracts(7)

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(1 of 2)
(+) Widely distributed in aquatic
environments, bacteria in the genus Vibrio are considered
autochthonous bacteria in marine and estuarine waters.
Capable of causing infection in humans, some Vibrio
species have been isolated from a variety of intestinal and
extraintestinal sites. Specifically, this bacteria can cause
gastroenteritis in humans. PCR method is the conventional
means of detecting the target with the tdh gene or trh gene,
i n whi ch the speci fi ci ty i n the chromosome of V.
parahaemolyticus gene of chitinase can be used as the
detection marker. However, this method is inefficient in
terms of detecting the target with the tdh gene or trh gene.
The inability to detect the target with the tdh gene or trh
gene more efficiently will lead to a higher incidence of food
poisoning in humans from aquatic foods, with an especially
high incidence during the spring season.
(2 of 2)
Therefore, this work describes a novel PCR-based
molecular biology method to identify a particular gene of Vibrio
parahaemolyticus rapidly. This gene in a chromosome is
extracted from bacteria. Amplification of this gene is then
achieved by using the PCR method. Next, PCR-based assays
are made by preparing all of the genomic DNAs of the strains in
Tris-EDTA buffer (TE; pH 8.0)according to previous literature.
Additionally, the purity and the amount of DNA in each
preparation are estimated colorimetrically, with the DNAs
subsequently stored at 4C until further use. Furthermore,
whether germs can be found in seafood is determined using the
PCR method. Analysis results indicate that
the proposed PCR-based molecular biology method identifies
rapidly the presence of marine bacteria in the early stages.
In addition to providing quick results with a high
degree of sensitivity, the proposed method yields rapid and
accurate results to detect Vibrio parahaemolyticus in the
environment.
(1 of 2)
(+) Gene expression requires
that many proteins interact with a regulated element.
However, modulation of gene expression has not been
thoroughly investigated, making it impossible to determine
the period and location of gene expression. Nevertheless,
expression of the same gene in a diverse cell can be
regulated based on different expressions and, thus, cannot
be expressed 100%. The inability to thoroughly understand
the mechanism of distinct gene expression makes it
impossible to understand how this unique mechanism
affects embryo development. Therefore, this study
elucidates the mechanism of gene expression in a cell
owing to the importance of acquiring genes in a cell for
embryo development.
(2 of 2)
The regulatory role of a gene in the upstream as
a promoter and enhancer is analyzed by adopting
molecular cloning methods. The identified gene is then
linked with a regulating gene to clarify how gene
expression sites are regulated.
Analysis results clarify the mechanism of gene
expression in cells and regulation of tissue-specificity
genes. Importantly, results of this
study provide a valuable reference for efforts to
develop a therapeutic method of gene expression for
patients with genetic defects.
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