Writing Science Abstracts(9)

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(1 of 2)
(+) Antioxidants consume
oxygen and free radicals in large amounts in mammals.
Individuals exercising for extended periods can regulate the
antioxidant activity of their skeleton muscles, accentuating
the role that antioxidant plays in exercise. However,
identifying which antioxidant enzyme activities are induced
for a particular exercise is extremely difficult, with a
variation in effects of up to ten times. The inability to
identify a standardized means makes it impossible to
examine the exact role of antioxidant enzyme activity in
exercise. Therefore, this work describes a novel
method to assess the role of antioxidant enzyme activity in
which a power training protocol can determine exact levels
accurately.
(2 of 2)
Exactl y how power trai ni ng i nfl uences the
antioxidant enzyme activity of skeleton muscles is
determined. Variations among antioxidant enzyme methods
are then examined. Next, variations in power training
protocols are studied. Additionally, the discriminative
features of skeleton muscles are examined.
Experimental results indicate that the proposed model
determi nes accurately anti oxidant enzyme activity,
providing insight into how to increase muscular power.
Results of this study contribute to efforts
to manifest the antioxidant ability in power training, thus
increasing the rat skeleton muscles markedly, which has
significant physiological implications.
(1 of 2)
(+) Given that amylases that produce
maltotriose as the major end product from raw starch granules are
relatively rare, efforts have been underway to optimize the cultivation
conditions for producing this extracellular amylase by Thermobifida
fusca NTU22. This newly isolated strain is of relevant interest because
the strain produces an extracellular amylase that releases maltotriose
as the major end product from either soluble starch or raw starch
gr anul es. The commer ci al l y pr oduced f or m i n bul k f r om
microorganisms represents about 2533% of the global enzyme market.
Most _-amylases (EC 3.2.1.1, 1,4-_-d-glucan glucanohydrolyase)
produce glucose or maltose as the major product from starch.
Production of this extracellular amylase by maltotriose-producing -
amylase from Thermobifida fusca NTU22 can replace thermophilic
microorganisms. Given that thermophilic microorganisms are
good sources of thermostability and novel amylases have potential
industrial importance, this study examines the feasibility of producing
this extracellular amylase by maltotriose-producing-amylase from
Thermobifida fusca NTU22 substitute for thermophilic microorganisms.
(2 of 2)
A thermophilic actinomycete, Thermobifida fusca NTU22, is
isolated from compost soils collected in this study. A maltotriose-
producing -amylase from Thermobifida fusca NTU22 is then purified
from crude culture filtrate by ammonium sulfate fractionation,
Sephar ose CL- 6B and DEAE- Sephar ose CL- 6B col umn
chromatography. Next, the overall yield of the purified enzyme is
determined. Additionally, the purified enzyme can produce SDS-
polyacrylamide gel electrophoresis (SDS-PAGE).
Analysis results indicate that, to produce enzymes for enzymatic
degradation of renewable lignocellulose, a potent extracellular
lignocellulolytic enzyme-producing thermophilic actinomycete,
Thermobifida fusca NTU22 from compost soils collected in Taiwan can
be isolated. Results of the -amylase gene from hermobifida fusca
NTU22 provide further insight into the structure and the regulation of -
amylase production in this thermophilic actinomycete.
In addition to the commercial interest associated with producing
high yields of a specific maltooligosaccharide on degradation of starch
by -amylase, maltotriose has many excellent properties, including a
mild sweetness, an ability to store well in moisture, and anti-
retrogradation capabilities of starch in foodstuffs.
Further details can be found at
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