'Standardization of Chandra Kala Ras S 'Standardization of Chandra Kala Ras S

Evaluation of its Rakt Stambhana Evaluation of its Rakt Stambhana

property along with anjabar sharbat property along with anjabar sharbat
w.s.r. to asrgdar' w.s.r. to asrgdar'
Dr. Supriya Shukla, N.D. Scholar Dr. Supriya Shukla, N.D. Scholar
Department of Rasa Shastra S Bhaishajya Kalpana Department of Rasa Shastra S Bhaishajya Kalpana
NS !D OB]ECTES OF THE NS !D OB]ECTES OF THE
STUDY STUDY
Compilation Compilation of of various various references references of of Chandrakala Chandrakala Rasa Rasa
scattered scattered in in various various classical classical texts texts. .
To To ensure ensure the the preparation preparation of of safe safe and and efficacious efficacious
Chandrakala Chandrakala Rasa Rasa and and Sharbata Sharbata Anjabar Anjabar. .
To To prepare prepare and and process process pharmaceutically pharmaceutically Abhraka Abhraka
Bhasma, Bhasma, Tamra Tamra Bhasma, Bhasma, and and last last three three sanskara sanskara of of
Hingulottha Hingulottha Parada Parada. .
Analytical Analytical study study for for Standardization Standardization of of Chandrakala Chandrakala Rasa Rasa
according according to to parameters parameters laid laid down down by by CCRAS CCRAS. .
To To explore explore and and compare compare clinically clinically the the Rakta Rakta Stambhana Stambhana
property property of of Chandrakala Chandrakala Rasa, Rasa, Sharbata Sharbata Anjabar Anjabar and and
Tandulodaka Tandulodaka w w. .ss. .rr to to Asrigdara Asrigdara. .
%ELEE!CE !D NPO%T!CE OF P%ESE!T %ELEE!CE !D NPO%T!CE OF P%ESE!T
STUDY STUDY
study study is is not not planned planned earlier earlier comparing comparing the the effects effects of of
the the present present three three drugs drugs used used . .
As As the the appropriate appropriate parameters parameters for for standardization standardization of of
Chandrakala Chandrakala Rasa Rasa are are yet yet not not decided, decided, therefore therefore its its an an
attempt attempt to to contribute contribute in in the the further further research research in in this this area area
. .
An An attempt attempt is is made made to to establish establish clinically clinically efficacy efficacy of of
Chandrakala Chandrakala Rasa Rasa and and Sharbata Sharbata Anjabar, Anjabar, in in the the treatment treatment of of
excessive excessive uterine uterine bleeding bleeding. .
Clinical Clinical estimation estimation of of the the best best therapy therapy out out of of four four groups groups
prepared prepared by by utilizing utilizing three three formulations formulations. .
STANDERD!ZAT!ON STANDERD!ZAT!ON
Defined as the process of developing and agreeing upon Defined as the process of developing and agreeing upon
technical STANDARDS. technical STANDARDS.
STANDARD : !s a document that eastablishes uniform STANDARD : !s a document that eastablishes uniform
engineering or technical specifications, engineering or technical specifications,
criteria, methods, processes or practices. criteria, methods, processes or practices.
CHO!CE OF DRUC CHO!CE OF DRUC
Chandrakala Chandrakala Ras Ras is is effectively effectively used used in in the the treatment treatment of of Raktapitta Raktapitta
and and considered considered as as the the drug drug of of choice, choice, but but its its use use in in the the treatment treatment
of of Asrgdar Asrgdar is is not not very very popular popular in in spite spite of of the the fact, fact, that that both both the the
diseases diseases have have similar similar line line of of treatment treatment as as evidenced evidenced by by the the
reference reference present present in in Sushrut Sushrut Samhita Samhita: :
r#.;k Igr~lsIoU;kLreYiksinzoa IHk"kd~ r#.;k Igr~lsIoU;kLreYiksinzoa IHk"kd~
A A
iDrIiUkIo/kkusu ;Fkkor~ leqikpisr AA iDrIiUkIo/kkusu ;Fkkor~ leqikpisr AA
lq0'kk0 lq0'kk0 2(22 2(22
Hence Hence the the study study is is conducted conducted with with an an attempt attempt to to prove prove the the
practical practical implication implication as as well well as as clinical clinical efficacy efficacy of of Chandrakala Chandrakala Ras Ras
in in Asrgdar Asrgdar. .
ABOUT THE D!SEASE ABOUT THE D!SEASE
ASR!CDAR ASR!CDAR
Condition characterised by excessive flow of menstrual Condition characterised by excessive flow of menstrual
blood . blood .
!t refers to all types of abnormal uterine bleeding. !t refers to all types of abnormal uterine bleeding.
!n modern aspect it may be correlated to menorrhagia !n modern aspect it may be correlated to menorrhagia
and metrorrhagia. and metrorrhagia.
Nain pathology bieng the active or passive congestion, Nain pathology bieng the active or passive congestion,
which leads to increase in the amount of blood in uterus which leads to increase in the amount of blood in uterus
Nainly 4 types are described in classical texts, i.e v, P, K, Nainly 4 types are described in classical texts, i.e v, P, K,
S. S.
Nain clinical symptoms include ex.bleeding p/v, body Nain clinical symptoms include ex.bleeding p/v, body
ache, pain during menstruation. ache, pain during menstruation.
Differential diagnosis of the disease Differential diagnosis of the disease
Adhoga rakta pitta Adhoga rakta pitta
Pittala yonivyapada Pittala yonivyapada
Rakta yoni Rakta yoni
Putraghni yoni Putraghni yoni
Yoni arsha and yoni kanda Yoni arsha and yoni kanda
NENSTRUAT!ON NENSTRUAT!ON
Def: !t's a normal periodic uterine bleeding, Def: !t's a normal periodic uterine bleeding,
physiological function occurring only in physiological function occurring only in
female primates. female primates.
Normal Aspects : Normal Aspects :
Age of onset Age of onset -- 11 to 1Syrs 11 to 1Syrs
Termination Termination -- 4S to SSyrs(av.S0yrs) 4S to SSyrs(av.S0yrs)
Cycle Cycle -- 28days (21 to 3S) 28days (21 to 3S)
Duration Duration -- 3 to Sdays 3 to Sdays
Flow Flow -- 20 to 80ml 20 to 80ml
Pharmaceutical study Pharmaceutical study
Abharak shodhan Abharak shodhan
Ref RefAF! PART 1 AF! PART 1 -- 18:1,(R.T. 10/ 20) 18:1,(R.T. 10/ 20)
Naterial required Naterial required
krishna vajra krishna vajra abhraka abhraka 1.4 kg 1.4 kg
triphala 10.Skg triphala 10.Skg
water 84 lt water 84 lt
Procedure Procedurered hot abhraka red hot abhrakaquenched in quenched in
triphala qwatha triphala qwathaprocess repeated 7 times process repeated 7 times
Result Result Yield Yield 1.701kg 1.701kg
Weight gain Weight gain 21.S% 21.S%
Dhanyaabhrikarana Dhanyaabhrikarana
reI: AFI.Part reI: AFI.Part- -1,18:1(A.P 2/113 1,18:1(A.P 2/113- -114) 114)
Mat.req Mat.req- -
shuddha abhraka shuddha abhraka- -1.701 kg 1.701 kg
shali dhanya shali dhanya- -425.25 gm 425.25 gm
kanii kanii - -5lits 5lits
Procedure Procedure- - abhrakapaddy in iute bag abhrakapaddy in iute bag- -immersed in kanii Ior 3 immersed in kanii Ior 3
days days vigrous rubbing oI bag in kanii vigrous rubbing oI bag in kanii- -abhraka collected by abhraka collected by
settling and drying settling and drying
Result Result- - yield yield- -1550 gms 1550 gms
loss loss- -151gm(8.8) 151gm(8.8)
Abhraka Narana Abhraka Narana
ref: ref: Ra.Chin 4/18 Ra.Chin 4/18- -20 20
Mat.req: Mat.req:
shu.abhraka shu.abhraka1SS0gms 1SS0gms
Arka Ksheera Arka Ksheera- -10lits 500ml 10lits 500ml
Vata iata Kwatha Vata iata Kwatha- -4.5 lits 4.5 lits
Procedure Procedurearka ksheera bhawana arka ksheera bhawana
chakrika chakrikasharava samputa sharava samputa gajaputa gajaputa
process repeated 7times process repeated 7timeslast 3 puta with last 3 puta with
vata jata kwatha. vata jata kwatha.
Result Resultyield after 10 yield after 10
th th
puta puta1S10gm 1S10gm
loss loss30gms 30gms
Amritikarana of Abhraka Amritikarana of Abhraka
Ref:Ra.Chin 4/32 Ref:Ra.Chin 4/3233 33
Nat.req: abhraka bhasma Nat.req: abhraka bhasma1.Sgms 1.Sgms
goghrita goghrita1.2kg 1.2kg
triphala quatha triphala quatha2.4lits 2.4lits
Procedure Procedure above 3in 10:8:16 ratio above 3in 10:8:16 ratio
subjected to mild heat subjected to mild heattill ghrita till ghrita
digested digesteddark brown granular powder dark brown granular powder
obtained. obtained.
Result Result yield yield2337gms 2337gms
gain gain837gms(S3.8%) 837gms(S3.8%)
Tamra Shodhana Tamra Shodhana
SANANYA SHODHANA SANANYA SHODHANA
Ref:AF!,Part Ref:AF!,Part1,18:S,Sh.Sm.Nk 11/2 1,18:S,Sh.Sm.Nk 11/233
Nat req Nat req
copper wire copper wire730gms 730gms
tila taila tila taila4.Slits 4.Slits
takra takra4.Slits 4.Slits
kanji kanji4.Slits 4.Slits
gomutra gomutra4.Slits 4.Slits
kulthi kwatha kulthi kwatha4.Slits 4.Slits
Procedure Procedurered hot wires red hot wiresquenched in above liq.media quenched in above liq.media
one by one one by oneprocess to be repeated 3 times. process to be repeated 3 times.
Result Resultyield yield770gm 770gm
loss loss20gm(2.S%) 20gm(2.S%)
v!SHESH SHODHANA v!SHESH SHODHANA
Ref:AF!,Part1,18:S,A.P 3/118 Ref:AF!,Part1,18:S,A.P 3/118113 113
Nat.Req Nat.Req
samanya shodhita tamra samanya shodhita tamraS00gms S00gms
snuhi ksheera snuhi ksheera2S0ml 2S0ml
arka ksheera arka ksheera2S0ml 2S0ml
saindhava saindhavaS00gms S00gms
nirgundi qwatha nirgundi qwatha3lits 3lits
Procedure Proceduretamra smeared with tamra smeared with
paste(snuhi+arka+saindhava) paste(snuhi+arka+saindhava)heated till redhot heated till redhot
quenched in nirgundi qwath quenched in nirgundi qwathprocess repeated 3 times. process repeated 3 times.
Result Result yield yield6S1.Sgms 6S1.Sgms
gain gain1S1.S(30.S%) 1S1.S(30.S%)
Tamra Narana Tamra Narana
!ncludes 4 procedure !ncludes 4 procedure
1.Shodhana 1.Shodhana a.samanya a.samanya
b.vishesh b.vishesh
2.jarana 2.jarana
3.marana 3.marana
4.amritikarana 4.amritikarana
Tamra Narana Tamra Narana
Ref:AF!,Part1,18:S,Sh.Sm.Nk 11/20 Ref:AF!,Part1,18:S,Sh.Sm.Nk 11/2030 30
Step wise processing involved : Step wise processing involved :
a. gandhaka jarana a. gandhaka jarana
b. dhatu pisti formation b. dhatu pisti formation
c. golaka formation covering with gandhaka and c. golaka formation covering with gandhaka and
punernava kalka punernava kalka
d. puta paka for 12hrs d. puta paka for 12hrs
e. putapaka in gajaputa thrice e. putapaka in gajaputa thrice
f. amritikarana in surana kanda f. amritikarana in surana kanda
Tamra Narana Tamra Narana
Swedana of copper wires in amla drava Swedana of copper wires in amla drava
Nat.Req Nat.Req shu.tamra shu.tamraS00gms S00gms
kanji kanjiS.6lits S.6lits
Procedure Procedureheating wires in kanji on 60 heating wires in kanji on 6070 70
degrees degreeswashed with warm water washed with warm water
dried. dried.
Result Result
yield yield 487.40 487.40
loss loss12.60(2.S%) 12.60(2.S%)
Tamra Narana Tamra Narana
]arana ]arana
Nat.Req Nat.Req
tamra after amla paka tamra after amla paka487.40gms 487.40gms
shu.gandhaka shu.gandhakaS78gms S78gms
Result Result yield: 814.40gms yield: 814.40gms
Dhatu Pishti Dhatu Pishti
Nat.Req Nat.Req
shu.sans.parada shu.sans.parada12Sgms 12Sgms
jarita tamra jarita tamra 814.40 814.40
Result Result
yield: 304gms yield: 304gms
loss: 3Sgms loss: 3Sgms
Tamra Narana Tamra Narana
Colaka formation for 12hrs paka Colaka formation for 12hrs paka
Nat.req Nat.req
dhatu pishti dhatu pishti304gms 304gms
neembu swarasa neembu swarasaS00ml S00ml
shu.gandhaka shu.gandhaka1000gms 1000gms
punernava punernavaS00gms S00gms
gum acacia gum acacia100gms 100gms
saindhava saindhava1S0gms 1S0gms
Result Result
yield: 670gms yield: 670gms
Tamra Narana Tamra Narana
Putapaka 1 Putapaka 1
st st
Nat.Req Nat.Req
tamra after 12hrs paka tamra after 12hrs paka640gms 640gms
shu.gandhaka shu.gandhaka320(1/2 0f wt of tamra) 320(1/2 0f wt of tamra)
suranakand swarasa suranakand swarasa22Sml 22Sml
go ghrita go ghrita67gms 67gms
Procedure Procedure bhavana of surana swarasa bhavana of surana swarasa
golaka formed golaka formedgandhaka smeared gandhaka smearedgajaputa. gajaputa.
Result Result
yield after 3 yield after 3
rd rd
puta putaS88gms S88gms
loss loss 1Sgms(2.4%) 1Sgms(2.4%)
Result of three putapaka at a glance Result of three putapaka at a glance
$.No.
Wt oI cowdung
cakes
Wt beIore puta paka Wt aIter putapaka
Gain/los
s
Kalka
Tamra
golaka
Kalka
Tamra
golaka
Puta I 4.722 kg
185.80
gm
640 gm 42.0 gm 572gm -68gms
Puta II 4.288 kg 42 gm 562 gm
36.80
gm
613gm 51gms
Puta III 6.999 kg. 36.80 gm 603gm
31.50
gm
588gm -15gms
Amritikarana of Tamra Bhasma Amritikarana of Tamra Bhasma
Ref:AF!,Part1,18:S,R.T 17/40 Ref:AF!,Part1,18:S,R.T 17/4041 41
Nat.Req Nat.Req
tamra bhasma tamra bhasmaS78gms S78gms
shu.gandhaka shu.gandhaka283(1/2 of wt of tamra) 283(1/2 of wt of tamra)
neembu swarasa neembu swarasa200ml 200ml
surana kanda surana kanda2 nos.(Skg) 2 nos.(Skg)
Result Result yield:706.40gms yield:706.40gms
gain:107.40(18%) gain:107.40(18%)
Hingulotha Parada Hingulotha Parada
Ref:AF!,Part1,App.2,Rm. 1/16 Ref:AF!,Part1,App.2,Rm. 1/1617 17
Nat.Req Nat.Req
hingula hingula17S0gms 17S0gms
neembu swarasa neembu swarasaq.s q.s
Procedure Procedureshodhana with neebu swarasa shodhana with neebu swarasa
kept in U.P yantra kept in U.P yantrativra agni 6hrs tivra agni 6hrsblack black
powder in U.pot powder in U.potsieved thru 4 layer cloth sieved thru 4 layer cloth
globules obtained. globules obtained.
Result Result yield of Hg: 631gms(36%) yield of Hg: 631gms(36%)
Parada Sanskara Parada Sanskara
BODHANA BODHANA
Ref:AF!,Part1,App.2,R.Chu 4/88 Ref:AF!,Part1,App.2,R.Chu 4/88
Nat.Req Nat.Req
hingulotha parada hingulotha paradaS00gms S00gms
saindhava saindhava1S6.2Sgms 1S6.2Sgms
water water7S0ml 7S0ml
vatapatra vatapatraas required as required
Result Result yield:467gms yield:467gms
loss:33gms(6.6%) loss:33gms(6.6%)
Parada Sanskara Parada Sanskara
N!YANANA N!YANANA
Ref:AF!,Part1,App.2,R.H.T 2/10 Ref:AF!,Part1,App.2,R.H.T 2/10
Nat.Req Nat.Req
Bodhita parada Bodhita parada23gms 23gms
lasuna lasuna23gms 23gms
nagvalli nagvalli23gms 23gms
ambuja ambuja23gms 23gms
karkoti karkotinot found not found
markava markava23gms 23gms
chincha phala chincha phala23gms 23gms
kanji kanjiq.s q.s
Result Result
yield:4S6.60gms yield:4S6.60gms
loss:10.4gms(2.2%) loss:10.4gms(2.2%)
Parada Sanskara Parada Sanskara
DEEPANA DEEPANA
Ref:AF!,Part2,App.2,R.H.T 2/11 Ref:AF!,Part2,App.2,R.H.T 2/11
Nat.Req Nat.Req
niyamita parada niyamita parada4S6.60gms 4S6.60gms
sphatika sphatika28.S0gms 28.S0gms
shu.kasisa shu.kasisa28.S0gms 28.S0gms
shu.tankana shu.tankana28.S0gms 28.S0gms
maricha maricha28.S0gms 28.S0gms
lavana lavana28.S0gms 28.S0gms
asuri asuri28.S0gms 28.S0gms
shigru beej shigru beej28.S0gms 28.S0gms
kanji kanjiq.s q.s
Result Result
yield yield4S4.40gms 4S4.40gms
loss loss2.20gmss 2.20gmss
PREPARAT!ON OF CHANDRAKALA RASA PREPARAT!ON OF CHANDRAKALA RASA
Ref:AF!,Part1,20:1S,Y.R,mk.chi,sh.74 Ref:AF!,Part1,20:1S,Y.R,mk.chi,sh.7482gms 82gms
Nat.req Nat.req
shu.sans.parada shu.sans.parada410gms 410gms
tamra bhasma tamra bhasma410gms 410gms
abhraka bhasma abhraka bhasma410gms 410gms
shu.gandhaka shu.gandhaka820gms 820gms
Bhawana Drugs Bhawana Drugs
musta kwatha musta kwatha14lits 14lits
dadima swarasa dadima swarasa14lits 14lits
durva kwatha durva kwatha14lits 14lits
ketaki kwatha ketaki kwatha14lits 14lits
kumari kwatha kumari kwatha14lits 14lits
sahdevi kwatha sahdevi kwatha14lits 14lits
parpata kwatha parpata kwatha14lits 14lits
Ramsheetalika kwatha Ramsheetalika kwatha14lits 14lits
shatavari kwatha shatavari kwatha14lits 14lits
draksha kwatha draksha kwatha14lits 14lits
Prakshepa Drugs Prakshepa Drugs
tikta tikta400gms 400gms
guduchi satva guduchi satva400gms 400gms
parpata parpata400gms 400gms
ushira ushira400gms 400gms
madhavi madhavi400gms 400gms
sh.chandana sh.chandana400gms 400gms
sh.sariva sh.sariva400gms 400gms
PREPARAT!ON OF CHANDRAKALA RASA PREPARAT!ON OF CHANDRAKALA RASA
Table showing wt of extract value added by decoctions Table showing wt of extract value added by decoctions
Name Name % Extract % Extract Wt of 14lit qwatha Wt of 14lit qwatha
1.Nusta 1.Nusta 44 S60gm S60gm
2.Dadima 2.Dadima 14.6 14.6 2.044kg 2.044kg
3.Durva 3.Durva 22 280gm 280gm
4.Ketaki 4.Ketaki 33 420gm 420gm
S.Sahdevi S.Sahdevi 44 S60gm S60gm
6.Kumari 6.Kumari 3.1 3.1 434gm 434gm
7.Parpata 7.Parpata 44 S60gm S60gm
8.Ramsheetalika 8.Ramsheetalika 44 S60gm S60gm
3.Shatavari 3.Shatavari 77 2.380kg 2.380kg
10.draksha 10.draksha 41.3 41.3 S.782kg S.782kg
total total 87 87 13.S80kg 13.S80kg
Procedure Procedure
1.formation of dhatu kajjali and its division into 3 1.formation of dhatu kajjali and its division into 3
respective samples. respective samples.
2.seven bhawana of ten drugs each in form of 2.seven bhawana of ten drugs each in form of
rasakriya in the 3 samples. rasakriya in the 3 samples.
3.addition of prakshepa drugs in the 3 samples. 3.addition of prakshepa drugs in the 3 samples.
4.drying in shade to obtain req. consistensy. 4.drying in shade to obtain req. consistensy.
S.formation of pill using go ghrita. S.formation of pill using go ghrita.
Result and observation Result and observation
1.final yield after making pills 1.final yield after making pills
sample 1 sample 1
st st
S.346kg S.346kg
sample 2 sample 2
nd nd
S.336kg S.336kg
sample 3 sample 3
rd rd
S.400kg S.400kg
PREPARAT!ON OF CHANDRAKALA RASA PREPARAT!ON OF CHANDRAKALA RASA
Physical characteristics of pills in 3 samples Physical characteristics of pills in 3 samples
1.color 1.color -- black black
2.odour 2.odour -- sweet smell of its own kind sweet smell of its own kind
3.taste 3.taste -- mild sweet and mainly tikta mild sweet and mainly tikta
4.appearance 4.appearance -- smooth having sticky ,elastic nature smooth having sticky ,elastic nature
S.solubility S.solubility -- dissolves in water leaving behind residue dissolves in water leaving behind residue
6.Ph 6.Ph -- 6.S 6.S
Extract value added in the final product Extract value added in the final product
(A).Probable yield of chandrakala rasa : 18.380kg (A).Probable yield of chandrakala rasa : 18.380kg
wt. of ext. of bhawana drugs wt. of ext. of bhawana drugs 13.S80kg 13.S80kg
where wt of prakshepa drugs where wt of prakshepa drugs 2.8 kg 2.8 kg
wt of kajjali wt of kajjali 2kg 2kg
(B). Actual yield of chandrakala rasa obtained : 16.082kg (B). Actual yield of chandrakala rasa obtained : 16.082kg
App. Wt of ext. of bhawana drugs App. Wt of ext. of bhawana drugs 11.282kg 11.282kg
where wt of prakshepa drugs where wt of prakshepa drugs -- 2.8kg 2.8kg
wt of kajjali wt of kajjali 2kg 2kg
PREPARAT!ON OF CHANDRAKALA RASA PREPARAT!ON OF CHANDRAKALA RASA
Anjabar sharbata Anjabar sharbata
Ref: NFUN, vol.2, pg.21 Ref: NFUN, vol.2, pg.21
Nat.Req Nat.Req --
1.anjabar mula 1.anjabar mula -- 7kg 7kg
2.kharnoob shami 2.kharnoob shami -- Skg Skg
3.burada sandal surkh 3.burada sandal surkh -- 2kg 2kg
4.burada sandal safaid 4.burada sandal safaid -- 2kg 2kg
S.habb S.habbul ulaas aas -- 2kg 2kg
6.aab 6.aabeeahantab ahantab -- 100lits (Skg Fe turnings used) 100lits (Skg Fe turnings used)
7.quand sufaid 7.quand sufaid -- 7Skg 7Skg
Observation and Result Observation and Result
color color voilet voilet
taste taste madhur amla kashaya madhur amla kashaya
yield yield 8Slits 8Slits
Result Result
loss of wt = probable yield loss of wt = probable yield -- actual yield actual yield
= 18.380 = 18.380 -- 16.082 16.082
= 2.238kg = 2.238kg
% of loss = 12.S% % of loss = 12.S%
5alytical study 5alytical study
ANALYT!CAL STUDY ANALYT!CAL STUDY
Samples a5alysed Samples a5alysed
3 samples of chandrakala rasa 3 samples of chandrakala rasa
abhraka bhasma abhraka bhasma
tamra bhasma tamra bhasma
Parameters Parameters
Organoleptic characters Organoleptic characters --
color, odour, taste, touch, varitaratva, color, odour, taste, touch, varitaratva,
rekhapurnatva, nishchandratva. rekhapurnatva, nishchandratva.
Psysicochemical Psysicochemical --
LOD, Av, A!A, ASE, WSE, DT. LOD, Av, A!A, ASE, WSE, DT.
Elemental assay Elemental assay --
AAS, XRD AAS, XRD
Others Others -- Nicrob. Limit test, carbohydrate estimation Nicrob. Limit test, carbohydrate estimation
Table showing Organoleptic characters of 3 samples
of Chandrakala rasa
S.No. Parameters Observation
S1 S 2 S 3
1 Colour lack lack lack
2 Odour $weet smell $weet smell $weet smell
3 Touch $oIt smooth,
compressible
$oIt smooth,
compressible
$oIt smooth,
compressible
4 Taste Tikta,
madhur
Tikta,
madhur
Tikta,
madhur
5 pH 6.S 6.S 6.S 6.S 6.S 6.S
Table showing organoleptic characters of Abhraka
Bhasma in different stages of processing
S.
No
Parameters
After
shodhana
After Dhanya
-bhrikarma
After I
puta
After 10
th
puta
After
amritikarana
1 Colour lack Grayish black
Mostly
grey with
orange
patches
rick red CoIIee brown
2 Odour Odourless
Mild
unpleasing
odour
Odourless Colourless Odourless
3 Touch
Coarse
powder
moderately
Fine powder
Fine Very Iine Granular
4 Taste Astringent Astringent
Mild
$alty
Mild $alty Tasteless
5 Appearance $hiny $hiny $hiny
Diminishe
d shine
shine
dissappeared
6 Rekhapurnata
7 Variataratva partially
Table showing organoleptic characters of Tamra Bhasma
in different stages of processing
S.
No
Parameters After 1arana
After 12 hrs
pultapaka
After 3
rd
puta
After Amriti
karana
1 Colour lack lack lack lack
2 Odour Odourless Odourless Odourless Odourless
3 Touch
Coarse
powder
Moderately
Iine
Fine Very Iine
4 Taste
Metallic/
kashaya
Tasteless Tasteless Tasteless
5 Appearance Mild Haziness $hiny $hiny $hiny
6
Rekha
purnata

7 Varitaratva Partially -
8
Dadhi
Pareekesha
Marked blue
discolouration
$light bluish
discolouration
No
discolouration
No
discolouration
Table showing physicochemical parameters of Chandrakala rasa.
S.No. Parameters Sample 1 Sample -2 Sample 03 Mean
1 Description lack colour lack colour lack colour
2 Loss on drying 5.12 4.39 4.59 4.7
3 Water soluble Ash 12.59 14.43 13.66 13.56
4 Acid Insoluble Ash 2.51 3.14 2.36 2.67
5 Alcohol soluble
extractive
41.01 36.0 42.24 39.75
6 Total Ash 18.85 18.24 14.18 17.09
7 Disintegration time 18 22 min 18 20 min 18 21 min 20 min
8 Carbohydrate 44.11 40.82 45.0 43.31
9 $ulphur 2.25 3.04 3.55 2.95
10 Mercury 0.39 0.36 0.38 0.38
S.No. Parameters Sample 1 Sample -2 Sample 03 Mean
11 Copper 0.59 0.56 0.58 0.58
12 Iron 0.07 0.06 0.05 0.06
13 Lead 0.001 0.001 0.001 0.001
14 Arsenic 0.0003 0.0003 0.0003 0.0003
15 Magnesium 0.09 0.08 0.08 0.083
16 $odium 0.3 0.3 0.3 0.3
17 Potassium 1.45 1.46 1.45 1.45
18 Zinc 0.01 0.01 0.01 0.01
19 Microbial analysis
$taphylococcus Aureus
E. coli
$almonella spp.
Pseudomonas aeruginosa
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Absent
Table showing Elemental analysis of Abhraka Bhasma through
A.A.S.
S.No.
Test Parameter Results
1 Description lack Colour
2 $ulphur 1.23
3 Iron 0.47
4 Arsenic 0.0004
5 Lead Not detected
6 Zinc 0.097
7 Cadmium 0.0004
8 Aluminum Not detected
9 Magnesium 0.19
10 Manganese 0.05
11 Mercury 0.003
Table showing Elemental analysis of Tamra Bhasma through
A.A.S.
S.No.
Test Parameter Results
1 Description lack Colour
2 $ulphur 27.40
3 Copper 1.1
4 Mercury 0.23
5 Lead 0.02
6 Arsenic 0.006
7 $odium 0.001
8 Potassium 0.18
9 Zinc 0.004
10 Cadmium 0.00002
11 Iron 0.33
Cli5ical Study Cli5ical Study
Diag5ostic Criteria Diag5ostic Criteria
Clinical signs S symptomatology viz Clinical signs S symptomatology viz
Amount of flow of menstrual blood. Amount of flow of menstrual blood.
Duration of flow of menstrual blood. Duration of flow of menstrual blood.
Assessment of inter menstrual period Assessment of inter menstrual period
Body Ache (Angamarda) Body Ache (Angamarda)
Weakness (Dourbalya) Weakness (Dourbalya)
Pallor (Pandutva) Pallor (Pandutva)
Burning sensation in body (Daha) Burning sensation in body (Daha)
Protocol includes 37 patients in 4 different Protocol includes 37 patients in 4 different
groups using randomised single blind trial groups using randomised single blind trial
method. method.
Criteria for i5clusio5 Criteria for i5clusio5
Patient aged between 12 to S0yrs of life Patient aged between 12 to S0yrs of life
Cases of bleeding P/v specifically endometrial bleeding Cases of bleeding P/v specifically endometrial bleeding
Bleeding caused by DUB, fibroid, adenomyosis and P!D Bleeding caused by DUB, fibroid, adenomyosis and P!D
Lab invest. to rule out any organic or systemic disease Lab invest. to rule out any organic or systemic disease
USC USC
Blood Blood Hb%, TLC, DLC, ESR, CT, BT, RBS Hb%, TLC, DLC, ESR, CT, BT, RBS
Criteria Criteria for for exclusio5 exclusio5
Patients of Age < 12 years S above S0 yrs of life. Patients of Age < 12 years S above S0 yrs of life.
Patients suffering from severe anemia Hb < 4 gm % Patients suffering from severe anemia Hb < 4 gm %
Systemic cause Systemic cause
Severe hypertension Severe hypertension
Liver dysfunction Liver dysfunction
Hypo dysfunction Hypo dysfunction
Congestive cardiac failure. Congestive cardiac failure.
Patient having bulky ulterus more than 8 week size. Patient having bulky ulterus more than 8 week size.
Patient using !UCD. Patient using !UCD.
Patients having bleeding from sites other than uterus Patients having bleeding from sites other than uterus
Patients having coagulation disorder Patients having coagulation disorder))
POSOLOGY
Group
Drug
Administered
Dose Vehicle Time oI
administration
Duration oI
treatment
1 a)Chandrakala
Rasa
b)$harbata
Aniabar
1 gm
25 ml
Tandulodaka
Water
Empty stomach
twice a day
3 menstrual
cycle or
3 months.
2 a)Chandrakala
Rasa
1 gm Tanduladaka Empty stomach
twice a day
3 menstrual
cycle or
3 months.
3 a)$harbat
Aniabar
25 ml Water Empty stomach
twice a day
3 menstrual
cycle or
3 months.
4 a)Tandulodaka 200 ml Empty stomach
twice day
3 menstrual
cycle or
3 months.
Follow Up Study Follow Up Study
ll ll patie5ts patie5ts were were regularly regularly a5alyzed a5alyzed every every 1S 1S days days till till the the e5d e5d of of trial trial
CL!CL SSESSNE!T CL!CL SSESSNE!T
patients patients were were assessed assessed for for rakta rakta stambhana stambhana property property on on the the basis basis of of symptom symptom rating rating score score
AMOUNT OF FLOW
Intensity of bIeeding Grade
ess than 15 pads / cycle - 0
15 to 20 pads / cycle - 1
20 to 25 pads / cycle - 2
More than 25 / cycle - 3
DURATION OF FLOW OR MENSTRUAL PERIOD
Days of bIeeding Grade
ess than 6 days - 0
ess than 8 days - 1
8 to 9 days - 2
10 & More than 10 days - 3
INTER MENSTRUAL PERIOD
IntervaI between two periods / cycIe Grade
Once in 26 28 days - 0
Once in 21 25 days - 1
Once in 16 20 days - 2
up to 15 days or rregular - 3
BODY ACHE
Grading of pain Score
Occasionally on doing Heavy work - 0
After doing extra work - 1
After doing Routine work - 2
Even without Routine work - 3
PALLOR
Grading for paIIor Score
Normal - 0
Mild - 1
Moderate - 2
Severe - 3
BURNING SENSATION IN BODY (DAHA)
Grading for Burning sensation Score
No burning - 0
Occasional mild burning - 1
Often mild burning - 2
Severe burning - 3
TabIe No. 14 showing TotaI effect of therapy in 37 Patients
Criteria for Criteria for
assessme5t assessme5t
No. of Patients
Group I Group II Group III Group IV
No. No. No. No.
Marked Improvement 2 20 1 10 1 10 - -
Moderate Improvement 4 40 3 30 - - 2 28.57
Mild Improvement 4 40 4 40 6 60 4 57.14
No Improvement - - 2 20 3 30 1 14.29
TotaI effect of therapy in 37 patients (Subjective Symptoms)
Groups
Total Effects ()
Group I 69.6
Group II 63.2
Group III 46.8
Group IV 57.9
RESULT RESULT
Statistical analysis of 4 groups on subjectives parameters Statistical analysis of 4 groups on subjectives parameters
shows: shows:
CROUP 1 CROUP 1
ST ST
-- Nax relief in !NP(32.31%), 6 out of 7 symptoms were Nax relief in !NP(32.31%), 6 out of 7 symptoms were
relieved. relieved.
CROUP 2 CROUP 2
ND ND
Nax relief in BURN!NC(100%), S out of 7 symptoms Nax relief in BURN!NC(100%), S out of 7 symptoms
were relieved. were relieved.
CROUP 3 CROUP 3
RD RD
Nax relief in DURAT!ON(81.2S%), 3 out of 7 Nax relief in DURAT!ON(81.2S%), 3 out of 7
symptoms were cured. symptoms were cured.
CROUP 4 CROUP 4
TH TH
-- Nax relief in FLOW(78.S7%), 1 out of 7 was cured Nax relief in FLOW(78.S7%), 1 out of 7 was cured. .
!ntergroup comparision revealed !ntergroup comparision revealed NON S!CN!F!CANT NON S!CN!F!CANT result result
of all the parameters, except for body pain of all the parameters, except for body pain
Total effect of therapy in descending order: group 1 Total effect of therapy in descending order: group 1
st st
, ,
group 2 group 2
nd nd
group group
4th 4th
, group 3 , group 3
rd rd
. .
Discussion Discussion
Discussio5 o5 Co5ceptual study Discussio5 o5 Co5ceptual study
First First reI reI.. oI oI chandrakala chandrakala rasa rasa was was Iound Iound in in RR..RR..$ $ ( ( 13 13
th th
century) century)..
Chandrakala Chandrakala Rasa Rasa is is prepared prepared Iollowing Iollowing the the method method prescribed prescribed in in
AFI AFI with with required required changes changes in in the the $ $..O O..P P.. Iollowing Iollowing its its legal legal notice notice..
It It has has three three mains mains aspect aspect regarding regarding $ $..O O..P P.. which which are are Dhatu Dhatu kaiiali kaiiali
Iormation, Iormation, hawana hawana Iollowed Iollowed by by addition addition oI oI prakshepa prakshepa drugs drugs..
Addition Addition oI oI prakshepa, prakshepa, aIter aIter the the hawana hawana is is the the evidence evidence oI oI
rational rational scientiIic scientiIic approach approach as as this this makes makes handling handling && processing processing
easy,increases easy,increases cost cost eIIiciency, eIIiciency, saves saves labour labour and and time time..
Its Its an an ee..gg.. .. oI oI drug drug prepared prepared Iollowing Iollowing the the concept concept in in Ra Ra..Chin Chin
66//38 38- -39 39 ii..ee.. in in aa Iormulation Iormulation parada parada should should be be used used with with abhraka abhraka
and and lauha lauha Ior Ior DEHA DEHA ANDHA ANDHA..
A A total total oI oI 66 path path oI oI M M..chandrakala chandrakala rasa rasa and and 55 path path oI oI Chandrakala Chandrakala
rasa rasa were were Iound Iound..
Compilation Compilation oI oI 36 36 classical classical text text having having reIs reIs.. OI OI the the drug drug has has been been
done done ,, out out oI oI which which ------ ------ were were schedule schedule books books..
Discussion on pharmaceutical study Discussion on pharmaceutical study
Difficulty was faced in kajjali formation due to greasiness of sulphur and Difficulty was faced in kajjali formation due to greasiness of sulphur and
abhraka bhasma. abhraka bhasma.
Kajjali was not lustureless due to shine of tamra bhasma ,justified by Kajjali was not lustureless due to shine of tamra bhasma ,justified by
ref. of SACHANDRA tamra bhasma in R.Sam ref. of SACHANDRA tamra bhasma in R.Sam. .
Aspects regarding bhawana to maintain uniformity in procedure: Aspects regarding bhawana to maintain uniformity in procedure:
1. Equal amount of liq. By wt. of substance is sufficient for bhavana 1. Equal amount of liq. By wt. of substance is sufficient for bhavana
in case of metallic bhasmas. in case of metallic bhasmas.
2. Ouatha was made following ANUKALP vidhi for swarasa(1:8 2. Ouatha was made following ANUKALP vidhi for swarasa(1:8 ) )
and ratio of (1:4 and ratio of (1:4 -- ) for NR!DU dravya. ) for NR!DU dravya.
3. Bhawana was given in form of RASAKR!YA due to paucity of time 3. Bhawana was given in form of RASAKR!YA due to paucity of time
and to facilitate quick drying and to facilitate quick drying. .
Total increase in the final product was found to be 8 times the wt.of Total increase in the final product was found to be 8 times the wt.of
starting material (Dhatu kajjali starting material (Dhatu kajjali2kg) 2kg)
There was loss of 2.238kg in the final yield may be due to: There was loss of 2.238kg in the final yield may be due to:
1. improper settling of liq. Nedia. 1. improper settling of liq. Nedia.
2. error of calibration , measurement or calculation. 2. error of calibration , measurement or calculation.
3. handling loss. 3. handling loss.
PHYSICOCHEMICAL PHYSICOCHEMICALANALYSIS ANALYSIS
$amples $amples oI oI the the drug drug were were proved proved sterile sterile aIter aIter microbial microbial
analysis,may analysis,may be be due due to to bacteriocidal bacteriocidal or or bacteriostatic bacteriostatic property property
inherent inherent in in herbomineral herbomineral Iormulations Iormulations..
Values Values oI oI LOD LOD ( (44..77 w/w), w/w), AV AV ( (17 17..09 09 w/w) w/w) and and AIA AIA
( (22..67 67 w/w) w/w) lies lies with with in in the the reI reI.. range range in in the the monograph monograph oI oI
DRUG DRUG as as per per CCRA$ CCRA$..
ELEMENTAL ELEMENTALANALYSIS ANALYSIS
Lead Lead is is Iound Iound to to be be present present in in the the permissible permissible limits limits and and
negligible negligible increase increase ( (11..47 47ppm) ppm) was was observed observed in in range range oI oI
arsenic arsenic in in samples samples oI oI ckl ckl rasa rasa..
Apart Apart Irom Irom mercury mercury and and sulphur, sulphur, presence presence oI oI other other elements elements
may may be be contributed contributed by by the the decoction decoction oI oI herbal herbal drugs drugs
Following Following elements elements were were present present in in the the samples samples oI oI tamra tamra as as
well well as as abhraka abhraka bhasma bhasma $, $, Fe, Fe, As, As, Zn, Zn, Cd, Cd, Hg Hg..
Elements Elements like like Mg Mg and and Mn Mn were were Iound Iound only only in in abhraka abhraka bhasma bhasma
were were as as Cu, Cu, Na Na and and K K only only in in tamra tamra bhasma bhasma..
XRD XRD analysis analysis oI oI chandrakala chandrakala rasa rasa:: sulphur( sulphur(70 70 ), ), Hg$( Hg$(20 20 ), ),
Cu$( Cu$(10 10 ) )..
Discussio5 O5 5alytical Study Discussio5 O5 5alytical Study
Discussio5 o5 Cli5ical study Discussio5 o5 Cli5ical study
On comparing the eIIectiveness oI the Iour Groups it was Iound On comparing the eIIectiveness oI the Iour Groups it was Iound
that the relieI in symptoms was clinically signiIicant in all the Iour that the relieI in symptoms was clinically signiIicant in all the Iour
Groups but statistically it was proved non Groups but statistically it was proved non- -signiIicant, this may be signiIicant, this may be
attributed to the Iact that although random sampling was done but attributed to the Iact that although random sampling was done but
incidentally, most oI the cases with severe underlying pathology incidentally, most oI the cases with severe underlying pathology
were Grouped under Group I and also due to small sample size in were Grouped under Group I and also due to small sample size in
the present study. the present study.
II the eIIect oI trial drugs on subiective symptoms is summarized, II the eIIect oI trial drugs on subiective symptoms is summarized,
it can be inIerred that the drugs in Group I (69.6) and Group II it can be inIerred that the drugs in Group I (69.6) and Group II
(63.2) are better remedy in treating the disease condition, (63.2) are better remedy in treating the disease condition,
although there is not much variation in the percentage oI change in although there is not much variation in the percentage oI change in
the two therapies, signiIies that the role oI Aniabar $harbat is not the two therapies, signiIies that the role oI Aniabar $harbat is not
much signiIicant in treating the disease. Further it implies that much signiIicant in treating the disease. Further it implies that
Tandulodaka in the Iorm oI Anupana as well as in the Iorm oI Tandulodaka in the Iorm oI Anupana as well as in the Iorm oI
single drug produced remarkable results. single drug produced remarkable results.
NODE OF ACT!ON NODE OF ACT!ON
Pharmacokinetic depends on panchmahabhuta Pharmacokinetic depends on panchmahabhuta
configuration,dealing with S aspects:rasa, guna, configuration,dealing with S aspects:rasa, guna,
veerya, vipaka and prabhava. veerya, vipaka and prabhava.
Action of compound formulation action depends on Action of compound formulation action depends on
factor: factor:
a. prakriti samsamveta or vikritivisham samveta a. prakriti samsamveta or vikritivisham samveta
b. incompatibilities like mana, guna and karma b. incompatibilities like mana, guna and karma
virrudha. virrudha.
c. samnya pratyarabdha or vichitra pratyarabdha. c. samnya pratyarabdha or vichitra pratyarabdha.
Kramana (anupana) brings about quick action and Kramana (anupana) brings about quick action and
increases bioavailability of the drug. increases bioavailability of the drug.
Dhatu kajjali ,as a chief content possess rasayana Dhatu kajjali ,as a chief content possess rasayana
property and acts as yogavahi. property and acts as yogavahi.
NODE OF ACT!ON NODE OF ACT!ON
AYUvED!C ATTR!BUTES OF CHANDRAKALA RASA AYUvED!C ATTR!BUTES OF CHANDRAKALA RASA
Rasa Rasa madhura, tikta, kashya madhura, tikta, kashya
Cuna Cuna -- laghu, snigdha laghu, snigdha
veerya veerya -- sheeta sheeta
vipaka vipaka -- madhura madhura
Karma Karma -- rasayana, yogavahi, deepana, rasayana, yogavahi, deepana,
pachana, mootrajanana, raktapittahara, rakt pachana, mootrajanana, raktapittahara, rakt
shodhaka, daha prashmana, rakta vardhaka.s shodhaka, daha prashmana, rakta vardhaka.s
SANPRAPT! CHATAKA SANPRAPT! CHATAKA
Dosha Dosha -- pitta and vata pitta and vata
Dushya Dushya -- rasa and rajah rasa and rajah
Adhisthana Adhisthana -- garbhashaya garbhashaya
Shrotusa Shrotusa -- artavavahi shrotusa artavavahi shrotusa
Dushti prakrana Dushti prakrana -- vimarga gamana vimarga gamana
atipravritti atipravritti
Roga marga Roga marga -- abyantara abyantara
NODE OF ACT!ON NODE OF ACT!ON
DOSHA DOSHA
vATA vATA -- madhur vipaka, snigdha and rasayana guna madhur vipaka, snigdha and rasayana guna
P!TTA P!TTA sheeta veerya sheeta veerya
DUSHYA DUSHYA
RA]AH RA]AH -- rakta shodhaka, raktaprasadaka drugs rakta shodhaka, raktaprasadaka drugs
RASA RASA -- deepana pachana drugs deepana pachana drugs
AD!STHANA ,SHROTUSA AD!STHANA ,SHROTUSA
CARBHASHAYA AND RA]OvAH! SHROTUSA CARBHASHAYA AND RA]OvAH! SHROTUSA -- shothahara, shothahara,
ropana, lekhana, sandhaniya, vranaropana, laghu guna. ropana, lekhana, sandhaniya, vranaropana, laghu guna.
DUSHT! PRAKRANA DUSHT! PRAKRANA
AT!PRAvR!TT! AT!PRAvR!TT! -- Rakt stambhaka drugs, rakt vardhaka Rakt stambhaka drugs, rakt vardhaka
Co5clusio5 Co5clusio5
1. Iirst description oI chandrakala rasa Iound in
R.R.$ (13th Century)
2. medicine should be manuIactured implying
GMP and $OP to prevent contamination oI any
sort.
3. analytical study oI chandrakala rasa revealed
the uniIormity oI the procedures in the three
samples .
4. Absence oI live pathogen in samples oI drug
proves the acteriostatic, acteriosidal eIIect oI
Herbominaral Iormulation
5. Increase oI Iinal product is estimated to be eight 5. Increase oI Iinal product is estimated to be eight
times the weight oI dhatu kaiiali. times the weight oI dhatu kaiiali.
6. All three Ayurvedic preparations did not impart any 6. All three Ayurvedic preparations did not impart any
side eIIect, esp. chandrakala rasa . side eIIect, esp. chandrakala rasa .
7. Clinical $tudy shows haemostatic potential oI all the 7. Clinical $tudy shows haemostatic potential oI all the
drugs in Iour groups with better results in group I drugs in Iour groups with better results in group I
and Group II . and Group II .
8. Combination oI drugs in Group I proved to be the 8. Combination oI drugs in Group I proved to be the
best remedy Ior treating the symptoms oI Asrigdara best remedy Ior treating the symptoms oI Asrigdara
as 6 out oI 7 were cured. as 6 out oI 7 were cured.
9. All 3 trial drugs esp. chandrakala rasa was well 9. All 3 trial drugs esp. chandrakala rasa was well
tolerated at the given dose ,with out any undesirable tolerated at the given dose ,with out any undesirable
side eIIect. side eIIect.
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