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Soo-Chen Cheng
RNA Processing
Degradation
Overview of mRNA Processing in Eukaryotes
Ribonucleoprotein Complexes
In nucleus
pre-mRNAs form hnRNPs for heterogeneous
nuclear ribonucleoprotein particles.
In cytoplasm
mRNAs form mRNPs.
• RNA Editing
RNA Splicing
RNA Splicing
tRNA Pre-tRNA
Structure of tRNA and pre-tRNA
tRNA Pre-tRNA
Splicing of pre-tRNA
tRNA Endonuclease tRNA Ligase
Consensus Sequences around
5’ and 3’ Splice Sites of Pre-mRNA
Major
AT-AC
Chemistry of the Pre-mRNA Splicing Reaction
Factors Involved in Pre-mRNA Splicing
RNA Components
Spliceosomal snRNAs
Lsm binding
U6 U5
U1 U2
U4
Sm binding Sm binding
Sm binding Sm binding
Lsm
Starket et al. Nature 409, 539-541 (2001) Collins et al. JMB 309, 915-923 (2001)
Base Pairing between U1, U2 and Pre-mRNA
Early in the Splicing Reaction
Factors Involved in Pre-mRNA Splicing
RNA Components
Factors Involved in Pre-mRNA Splicing
RNA Components
SR proteins
Others
SR Proteins in mRNA Splicing
The Structure of Group I and Group II Introns
Group I Group II
Splicing by Two Transesterification Reactions
Essence of Group I Introns
Mg2+
3’-End Formation of RNA
• Polyadenylation of mRNAs
• Histone mRNAs
• U-snRNAs
Similar 3’ Processing of Major Matazoan RNA Species
Model for Cleavage and Polyadenylation of pre-mRNAs
3’-End Formation of Histone mRNA
Stem Loop
• The cleavage reaction requires U7 snRNP Binding Protein
• Processing of pre-rRNA
• Modification
EM of Pre-rRNA Transcription Units from
Nucleolus of a frog oocyte
Processing of Pre-rRNA and Assembly of Ribosomes
in Higher Eukaryotes
Maturation of the Ribosome
Decatur, W. A. and Fournier,M. J., 2003, J. Biol. Chem. 278. 695-698.
Modification of rRNA Mediated by snoRNPs
Kiss, T., 2002, Cell 109, 145-148.
RNA Editing
Comparison of the T. brucei COIII mRNA with the
Corresponding Mitochondrial DNA Sequence