Cell WaLer oLenLlal

48lC61
WaLer oLenLlal
W 1he relaLlve Lendency of waLer Lo move from
one area Lo anoLher
W 8epresenLed by Lhe Creek leLLer + (sl)
W uue Lo osmosls gravlLy mechanlcal pressure
or maLrlx effecLs such as surface Lenslon
W + +
s
+ +
p
+ +
m
+ +
s
+ +
p
+ +
m
W + waLer poLenLlal of a cell + 0 aL
equlllbrlum
W +
s
soluLe or osmoLlc poLenLlal' always
negaLlve ln slgn
W +
p
pressure poLenLlal Lurgor pressure may
be () or (+) ln slgn
W +
m
maLrlc poLenLlal due Lo waLerblndlng
collolds ln Lhe cell negaLlve ln
ressure oLenLlal (+
p
)
W 1urgor pressure
W Maln pressure of Lhe cell conLenLs agalnsL
Lhe cell wall ln planL cells and bacLerla cells
W ueLermlned by Lhe waLer conLenL of
Lhe vacuole
W 8esulLlng from osmoLlc pressure
CsmoLlc ressure (+
s
)
W LxerLed by Lhe flow of waLer
Lhrough a semlpermeable membrane separaLlng
Lwo soluLlons wlLh dlfferenL concenLraLlons of
soluLe
W Arlses from Lhe Lendency of a pure solvenL Lo
move Lhrough a semlpermeable membrane
and lnLo a soluLlon conLalnlng a soluLe Lo
whlch Lhe membrane ls lmpermeable
ueLermlnaLlon of WaLer oLenLlal
1 LlghL dlfferenL sucrose concenLraLlons was prepared
and 73mL of each was dlspensed separaLely lnLo 130mL
beakers
2 Cyllnders were placed ln covered beakers Lo prevenL
Lhem from drylng ouL
3 Lach cyllnder was cuL ouL lnLo sllces of approxlmaLely
4cm and Lhe remalnlng poLaLoes were saved for arL ll
4 Cyllnders were bloLLed wlLh paper Lowels and welghed
ln seLs of Lwo WelghLs were recorded
3 Cne seL of cyllnders was puL ln each of Lhe beakers wlLh
sucrose soluLlons prepared prevlously
6 1he cyllnders were removed afLer an hour
and a half bloLLed wlLh paper Lowels and
welghed agaln 8esulLs were recorded
7 1he change ln welghL (AW) and Lhe percenL
change ln welghL (AW) were compuLed
8 1he molal concenLraLlon of sucrose LhaL glves
0 AW was deLermlned
9 1he +
s
ln bars of LhaL sucrose soluLlon was
compuLed
101he waLer poLenLlal of Lhe poLaLo was
deLermlned
ormulae
W Change ln WelghL
AW Wf Wl
W ercenL Change ln WelghL
AW x 100
W CsmoLlc ressure
+
s
ml81
ueLermlnaLlon of SoluLe oLenLlal +
s
1 Sap exLracLlon was done whereln s24 poLaLoes were peeled
chopped and placed ln a blender
2 1he blended poLaLoes were fllLered wlLh cheesecloLh
3 lL was Lhen sLored ln a covered beaker Cn Lhe freezlng polnL
deLermlnaLlon Peldenhaln LhermomeLer was lmmersed ln
crushed lcesalL baLh and Lhe readlngs were recorded
4 60mL sap was poured ln 230mL Lrlenmeyer flask wlLh magneLlc
sLlrrlng bar and Lhe LhermomeLer was Lhen lnserLed
3 1he flask was surrounded wlLh lcesalL baLh and was vlgorously
sLlrred When Lhe LemperaLure reads abouL a degree Celslus Lhe
LemperaLure was ready every 10 seconds
6 1he LemperaLure vs Llme was ploLLed 1he Lrue freezlng polnL 1
f

was deLermlned
7 LasLly Lhe pressure poLenLlal +
p
of Lhe cells of poLaLo was
calculaLed
ormulae
W 1rue reezlng olnL
1

00123
s
W SoluLe oLenLlal
+
s
122 1

8esulLs