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Study regulatory networks Drug discovery - mechanism of action Diagnostics - tumor diagnosis etc.
Explore microbial diversity Whole genome comparisons - genome evolution Identify DNA binding sites Diagnostics - tumor diagnosis
Identification of DNA regions bound by a protein. Compare a wild-type strain to a gene (DNA-binding protein). Do not need any prior knowledge of the sequence the protein binds.
Iyer et al. 2001 Nature, 409:533-538
DNA diagnostics
Uses of microarrays is cancer research and diagnosis.
2733 papers published on microarrays and cancer 1038 papers published on microarrays, gene expression, cancer diagnosis 0 since 1997
Interacting proteins - determining which proteins come together to form functional complexes.
Yeast 2-hybrid, affinity purification
Subcellular localization - site of localization can often provide clues to the function of a protein.
GFP tagging, immunofluorescence microscopy.
Protein activity - investigating the biochemical activities of proteins. Structural genomics - high-throughput analysis of the protein structure
From www.probes.com
Proteins
Primary structure - sequence
Searching databases Identifying functional domains
Western Blot
Determine the presence and level of a protein in a cell lysate. http://web.mit.edu/esgbio/www/rdna/rdna.ht ml - review of Northern, Western, and Southern blots.
Mass spectrometry
Many new techniques for protein detection and quantitation being developed.
Protein microarrays
Analysis of thousands of proteins at one time. Many different types
Antibody arrayed - detect many proteins Proteins arrayed - detect interacting proteins Proteins arrayed - detect interacting small molecules Etc.
Protein:protein interactions