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Contents
Nucleic Acids RNA & DNA
RNA Types
RNA Synthesis = Transcription Protein Synthesis = Translation DNA Replication Chromosomes
Nucleotides
sugar of DNA
sugar of RNA
Nucleosides
Nucleosides is a compound consisting of a nucleobase (base) bound to a ribose or deoxyribose sugar via a beta-glycosidic bond. Examples of nucleosides include cytidine, uridine, adenosine, guanosine, thymidine and inosine. Nucleosides can be phosphorylated (phosphate group is added) by specific kinases in the cell on 5 of the sugar (it's primary alcohol group -CH2-OH), producing nucleotides.
GC AT
GC AU
GC base pairing
Hydrogen bonds in red
http://en.wikipedia.org/wiki/Base_pair
Nucleobases
in blue Hydrogen bonds in red
http://www.synapses.co.uk/genetics/dnastruc.html
Nucleobases
in blue Hydrogen bonds in red
RNA Types
Basic purpose of RNA is to synthesize proteins
Step 1: Transcription RNAs are synthesized by DNA Step 2: Translation the RNAs synthesize the protein
rRNA
rRNA = ribosomal RNA
rRNA is the RNA component of the ribosome (ribosome = the cellular unit where all protein synthesis occurs) 1. rRNA provides a mechanism for decoding the transcript on mRNA into the 3 letter codons for amino acids and 2. rRNA provides peptidyl transferase that forms the peptide bond between the amino acids that the tRNAs bring in during translation
mRNA
mRNA = messenger RNA mRNA serves serves as a messenger that tells the cell (ribosomes) what protein to synthesize.
mRNA is synthesized in the nucleus and then binds to ribosome to start protein synthesis = translation.
The information contained in mRNA is used to "translate" the protein with specific sequences (it tells the tRNA what amino acid to bring in next in the sequence).
tRNA
tRNA = transfer RNA
tRNA transfer amino acids to the ribosomes during protein synthesis = translation.
The tRNA are formed in the nucleus and migrate into the cytoplasm. The assignment of the correct amino acid to each form of tRNA is crucial to translation. There are 20 different enzymes called aminoacyl-tRNA synthetases (aaRS) for the 20 amino acids that are incorporated into proteins.
Promotor - Transcription
In genetics, a promoter is a region of DNA that initiates (promotes) the transcription of a particular gene Promoters are located near the genes they regulate
(on the same strand and typically upstream towards the 5' region of the sense strand or towards the 3' region of the anti-sense strand).
In bacteria: The promoter is recognized by RNA polymerase and an associated sigma factor and synthesis is initiated.
In eukaryotes: The process is more complicated with 7 factors for binding RNA polymerase to the promoter in the transcription step called pre-initiation.
initiation
elongation
termination
http://en.wikipedia.org/wiki/Transcription_%28genetics%29
2.The transcribed mRNA goes to a ribosome and together with rRNA and tRNA synthesize the protein by correctly organizing the amino acids, i.e. by translating the transcribed genetic code.
Translation starts
1. the rRNA in the ribosome reads the 3 letter codon on the mRNA of the next amino acid and calls in the tRNA with the corresponding anticodon. 2. the tRNA with this anticodon has the corresponding amino acid attached and links to the mRNA. 3. the rRNA binds this new amino acid with the previous amino acid thus forming the protein. 4. the empty tRNA unlinks from the mRNA (repeat from 1).
http://en.wikipedia.org/wiki/TRNA
DNA Synthesis - 1
Polymerization is always in the 5' to 3' direction with new nucleotides being added to the 3' end. Because of this directional demand of the polymerization, one of the DNA strands is easily replicated in a continuous fashion (leading strand). the other strand can only be replicated in short segmental pieces (lagging strand). Short segments of complementary DNA, called Okazaki fragments, are produced, and these are linked together later by the enzyme ligase. The lagging strand is considered the new strand.
http://www2.chemistry.msu.edu/faculty/reusch/virttxtjml/nucacids.htm
DNA Synthesis - 2
Separation of a portion of the double helix takes place at a site called the replication fork. As replication of the separate strands occurs, the replication fork moves away and unwinds additional lengths of DNA (in diagram movement is to the left).
http://www2.chemistry.msu.edu/faculty/reusch/virttxtjml/nucacids.htm
http://www.pdbj.org/eprots/index_en.cgi?PDB%3A3BEP
Heliacase - Unwind
Helicases are a class of enzymes. They are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating 2 annealed nucleic acid strands (DNA, RNA,) using energy derived from ATP hydrolysis.
Restriction
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at specific recognition nucleotide sequences (with Type II restriction enzymes cutting double-stranded DNA) known as restriction sites. Such enzymes, found in bacteria and archaea, are thought to have evolved to provide a defense mechanism against invading viruses (bacteriophages).
Restriction
Chromosomes
The chromosomes of prokaryotes are circular and have one molecule DNA The chromosomes of eukaryotes are
long and linear packed in chromatin
Chromosomes - Eukaryotes
http://en.wikipedia.org/wiki/Chromosome
Chromatin
Chromatin is the combination of DNA and proteins that make up the nucleus of a cell. The primary functions of chromatin are to package DNA into a smaller volume to fit in the cell to strengthen the DNA to allow
mitosis and meiosis and prevent DNA damage, and
http://www2.chemistry.msu.edu/faculty/reusch/virttxtjml/nucacids.htm