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HISPIDA
CONTENTS
I. Introduction II. Background III. Materials and Method IV. Results and Discussion V. Conclusion
I. Introduction:
1. Actual Situation:
In the world: 246 mil. patients (2006). In Vietnam: 3.5 mil. patients (2003). 3rd place in top ten serious diseases.
I. Introduction:
2. Purpose:
Successfully establishment of type 2 diabetic animal model. Initially evaluate the ability to control plasma glucose of Benincasa Hispida.
II. Background:
1. Diabetes Definition
2. Chemicals Inducing Diabetes Disease 3. Benincasa Hispida
II. Background:
1. Diabetes Definition
Definition: Is a condition in which the body either doesnt produce enough, or doesnt properly response to, insulin. Classification:
Type 1: Results from the body's failure to produce insulin. Type 2: Results from Insulin resistance (cells fail to use insulin properly)
II. Background:
2. Chemicals Inducing Diabetes Disease
Streptozotocin (C8H15N3O7 - 265.221 g/mol)
Powder form Soluble in water; ketone, alcohol. Effect on cells (insulin producing cells)
STZ
II. Background:
2. Chemicals Inducing Diabetes Disease
Nicotinamide (C6H6N2O - 122.12 g/mol)
Prevents the activities of toxic agents inside the body. Helps in improving the function of cells. Lower the need of insulin.
NA
II. Background:
3. Benincasa Hispida
Long time used in traditional medicine Folk remedies using leaves, seeds, rinds, etc.
Benincasa Hispida
II. Background:
3. Benincasa Hispida
Classification:
Kingdom: Phylum: Class: Order: Family: Genus: Species: Plantae Magnoliophyta Magnoliopsida Cucurbitales Cucurbitaceae Benincasa B. Hispida
II. Background:
3. Benincasa Hispida
Ingredients:
Protein: Carbohydrate: Calcium: Phosphorus: Iron: Carotin: Vitamin B1: Vitamin B2: Vitamin PP: Vitamin C: Other elements 0.4g 2.4g 19mg 12mg 0.3mg 0.01mg 0.01mg 0.02mg 0.3mg 16mg
Instruments:
Autoclave Magnetic stirrer Spectrophotometer Laminar hood Incubator Microscopy
Eppendorf 1.5ml Forceps Scissors Micropipette 20 - 200 l Micropipette 100 - 1000 l Micropipette type 200l and 1000l Absorbance tissue Metal tray
The dose STZ (100mg/kg) with NA (120mg/kg) had successfully constructed type 2 diabetic mouse model.
V. Conclusion:
The dose STZ (100mg/kg) with NA (120mg/kg) has better effect on plasma glucose concentration of Mus musculus var. Albino and doesnt cause the damage in islets of Langerhans. The effect of Benincasa Hispida on type 2 diabetes has been demostrated but there should be much more time to observe clearly the effect of Benincasa Hispida on controlling plasma glucose ability.