Académique Documents
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COVER PAGE
Group Members
Azam A. Mohammed Laura Hyacinth Richard Nadram Dakota Malco Giselle Khan
Neila Nedd
Samantha Grant Germaine Gabriel
Introduction
According to the Millennium Development Goals, access
and a startling 10 % for domestic use, according to statistics from the Stockholm International Water Institute (SIWI).
contaminated?
Increase of diseases due to contaminated water such as
WHOs Vision
The WHO has stated:All people, whatever their stage of development and their social and economic conditions, have the right to have access to an adequate supply of safe drinking water.
The first WHO document dealing specifically with public
WASA
WASA was established on September 1965.
the people of Trinidad and Tobago and thereafter to be the center of Excellence within the water sector in the Caribbean.
WASA
Mission
-To deliver consistent, reliable, quality water and wastewater services. -To achieve sustainable financial self-sufficiency.
-To improve the organization's impact on the environment and pursue water security for the nation.
can erect public stand pipes at any place and remove any of the stand pipes at there discretion.
These public standpipes are used for filling of water for
vehicles. Most standpipes are lined with PVC since it is less reactive.
appearance.
When water has an unpleasant taste, odour &
appearance, it is natural for consumers to reject this water (i.e water that is discoloured or dirty.).
Taste and odour can be influenced by chlorination,
filtration.
For removing hydrogen sulphide aeration, granular
Bacteria
Protozoa
the consumption of water that is contaminated with human and animal excreta.
Contaminated water will most like show under a
drinking safety. Some organisms actually grow in pipe systems such as Legionella.
Burkholderia pseudomallei Campylobacter jejuni, C. coli Escherichia coli Francisella tularensis Legionella spp. Leptospira
Shigella spp.
Vibrio cholerae
Short
Short
Low
Low
High
Low
Adenoviruses Astroviruses
High High
Enteroviruses
Rotaviruses Sapoviruses
Long
Long Long
Moderate
Moderate Moderate
High
High High
potable water. The table is taken from the WHOs book Guidelines for Drinking Water.
Pathogen Persistence in water supplies May multiply Resistance to chlorine Low Relative infectivity High
Acanthamoeba spp.
Cyclospora cayetanensis
Entamoeba histolytica Naegleria fowleri
Long
Moderate May multiply
High
High Low
High
High Moderate
Clostridium perfringens
- Coliform bacteria
-
Chlorine Requirements
Chlorine is used as a universal disinfectant for water Both domestic and industrial potable water supplies are
problems.
Chlorine Requirements
Physical Properties of chlorine
a) b) c) d) e)
Boiling point-34.6 C Melting point-101 C Density -3.214 g/litre at 0 C and 101.3 kPa Vapour pressure-480 Pa at 0 C Water solubility-14.6 g/litre at 0 C
Chlorine Requirements
Types of chlorination -Breakpoint chlorination
-Marginal chlorination
-Super chlorination/dechlorination
Chlorine Requirements
Sodium hypochlorite solution is dosed using a positive-
dose oxidizes all the ammonia nitrogen in the water and leaves a suitable free residual chlorine available to protect the water .
Chlorine Requirements
When chlorine reacts with water, it forms hypochlorous
set by WHO is not exceeded as this may lead to a pungent taste (somewhat like bleach).
Chlorination can be attained by using liquefied chlorine
Chlorine Requirements
Superchlorination/dechlorination is the addition of a large
dose of chlorine for quick disinfection and chemical reaction, followed by reduction of excess free chlorine.
Chlorine also acts as an oxidant and can remove or
assist in the removal or chemical conversion of some compounds that can be removed from water by filtration.
Chloride- 250 mg/l Carbofuran -0.007 mg/l Carbon tetrachloride-0.004 mg/l Copper- 2 mg/l
2,4,6-Trichlorophenol-0.2 mg/l
Biological Requirements
Biological water standards addresses the presence of
is that it be free of (at low risk of containing) diseasecausing microorganisms. (WHO, 2002)
Biological Requirements
The term plankton is used in a broad sense by the World
Health Organization to include microscopic and nearmicroscopic free floating forms as well as minute attached organisms which develop on shores of lakes or reservoirs, or are attached to rocks or structures.
Biological Requirements
Most waterborne microbes are favorable, mostly as food
chain decomposers.
However some microbes may be pathogenic (potentially
disease causing).
The occurrence or lack of pathogens is very important,
Biological Requirements
Waterborne pathogens are placed in the general
Biological Requirements
Biological, or microscopic examination must be done. It should include qualitative analysis of the types of organism
contamination.
Biological Requirements
Not many pathogens are easily detected. Indicator organisms are a fundamental monitoring tool used
to measure both changes in water quality or conditions and the potential presence of hard-to-detect target pathogenic organisms.
An indicator organism provides evidence of the presence or
Biological Requirements
A fecal coliform (sometimes faecal coliform) is a
(e.g. Escherichia) as well as genera not of fecal origin (e.g. Enterobacter, Klebsiella, Citrobacter).
Biological Requirements
Some More Biological ContaminantsActinomycetes and fungi- Found in surface water sources and reservoirs. Cyanobacteria and algae-Can impede coagulation and filtration causing colour change in water. Can cause turbidity as well.
Invertebrates-Most of these invertebrates are microscopic such as Crangonyx pseudogracilis and Cyclops spp.
Biological Requirements
The current criteria of an ideal or preferred indicator of
fecal contamination have been defined and stated by WHO and other authorities.
According to these authorities the essential criteria of a
Biological Requirements
The indicator should be absent in unpolluted water and present
pathogenic ones.
The indicator should respond to natural environmental
conditions and water treatment processes in a manner similar to the pathogens of concern.
The indicator should be easy to isolate, identify and enumerate.
The test should be inexpensive thereby permitting numerous
analyses to be taken.
Biological Requirements
Biological quality of water determined by
Source Treatment processes Distribution
Biological Requirements
Faecal coliform, like other microorganisms, can usually be
coliforms.
Filtration with domestic filters can remove small animals.
Biological Requirements
Disinfection is a process that deactivates almost all
Biological Requirements
Biological Examination of water will aid in the following:-
a) Determine the causes of objectionable tastes and odours in water and controlling remedial treatments. b) Detect the organic pollution of water and contamination with toxic substances. c) Explain the causes of clogging of distribution pipes.
Radiological Requirements
Pollution of potable water by radioactive substances
possible.
The upper limits in potable water are:
alpha-emitters: 0.000000001 microcuries per millilitre beta-emitters: 0.00000001 microcuries per millilitre
Radiological Requirements
Standards 1 Bq = 1 disintegration per second.
seconds.
Radiological Requirements
Standards The following are the guideline levels of radio nuclides in
Hydrogen-3 Berellium-7
10 000 10 000
Carbon-14 Sodium-22
Phosphorus-32
100 100
100
Radiological Requirements
Radionuclides Phosphorus-33 Sulphur-35 Chlorine-36 Calcium-45 Calcium-47 Scandium-46 Scandium-47 Scandium-48 Vandium-48 Chromium-51 Manganese-52 Manganese-53 Manganese-54 Guideline level/Bq per Litre 1 000 100 100 100 100 100 100 100 100 10000 100 100000 100
Radiological Requirements
Radionuclides Iron-55 Iron-59 Cobalt-56 Cobalt-57 Cobalt-58 Nickel-59 Zinc-65 Germanium-71 Yttrium-90 Zirconium-93 Zirconium-95 Guideline level/Bq per Litre 1000 100 100 1000 100 1000 100 10000 100 100 100
accredited by the health authority or other agencies responsible for water quality.
Before granting such approval or accreditation to any
laboratory, the agency concerned should make certain that the facilities, quarters and equipment are adequate for the suitable working of the laboratory.
Laboratories must be certified by the Bureau of Standards.
b) equipped with suitable thermometers capable of registering accurately in the range 160-1 80C
Distinguishing E.coli, Aerobacter aerogenes and E. freudii. - Idole Test - Reagent: a) Dissolve 5 g p-dimethylaminobenzaldehyde in 75 ml of amyl alcohol.
b) Add 25 ml concentrated HCL c) Reagent appears yellow
Distinguishing E.coli, Aerobacter aerogenes and E. freudii. - Idole Test Procedure for Testing:a) Inoculate 5-ml portions of the medium. b) Incubate at 35-37C for 22 to 24 hours c) After incubation, add 0.2-0.3 ml of reagent and shake. d) Observe tubes after 10 minutes. e) A dark red colour in the amyl alcohol surface layer constitutes a positive indole test (remember colour was yellow initially).
To 1 ml of culture add 0.6 ml of a-naphthol solution and 0.2 ml of potassium hydroxide solution. A crimson to ruby colour forms in the mixture from 2 to 4 hours after adding the reagents. Results should be read not later than 4 hours after addition of the reagents.
b)
c)
Arsenic Testing: a) 5 ml of 24N sulfuric acid solution and 5 ml of concentrated nitric acid is added to portion of the sample containing from 0.002 to 0.040 mg of arsenic.
b) Cool and add 25 ml of distilled water and repeat to expel oxides of nitrogen. c) Dilute to 25 ml with distilled water.
Chemical Testing
Arsenic Testing: d) Dip the cotton roll into the lead acetate solution and put into the glass column in the Gutzeit generator. e) Add 5 ml of 24N sulfuric acid solution and cool To the 25 ml of the sample concentrate in the generator. f) Add 5 ml of potassium iodide solution, 4-5 drops of stannous chloride solution, and, 2-5 g of zinc. g) Connect as soon as possible, the absorption tube to the generator. Immerse the apparatus in a water-bath kept at 20-25C and leave for 1% hours. h) Amount of arsenic present can be estimated by means of a standard curve established in the laboratory.
solution and titrate with 4.0 g of disodium dihydrogen ethylenediamine-tetra-acetate dihydrate (sodium versenate) in 1 litre of distilled water
system. b) Samples are placed on petri dishes and observed under a light microscope for presence of plankton, small crustaceans and algae. c) A counting-cell is used to provide a known volume and area for microscopic examination and enumeration of organisms
Conclusion
The potable water standards used by WHO are used by WASA
microscopic organisms in minute quantities are used to prevent poisoning of the drinking water for human and consumption.
Laboratory procedures are used to accurately detect the correct
proportion of chemicals, microorganism and ensure that colour, odour and taste of water is acceptable.
Conclusion
All of the strict procedures are to be able to provide
References
World Health Organisation, (1958). International Standards for Drinking Water. Geneva: World Health Organization Publishing. World Health Organisation, (2011). Guidelines for drinking-water quality - 4th ed. Gutenberg: World Health Organization Publishing. World Health Organisation, (2008). Guidelines for drinking-water quality 3rd ed. Gutenberg: World Health Organization Publishing. Basch,P.F.(1999). Textbook of International Health. New York: Oxford University Press