A.

Mock

βlac

Infect: 3T12s MOI 0.5

Sort Sort
Rinse virus off after 1hr βlac+ βlac-

Day 0: Day 1: Infect 16hpi: Harvest and

Plate 3T12s 3T12s sort infected cells CyTOF

B. Mock Infected (n=1) βlac+ (n=4) βlac- (n=4)

20
20 20

0 0 0

-20
-20 -20
tSNE2

tSNE2

tSNE2
-20 0 20 -20
-20 00 20 20 -20
-20 0 0 20 20

tSNE1 tSNE1 tSNE1

C.
Viral Parameters Cell Surface Proteins Cell Cycle & Survival
vRCA pHistone H2AX Bst2 CD9 CD63 Ly6C MHCI Ki-67 pRb

0 6 0 8 0 4 1 6 0 6 0 6 0 2.5 0 4 0 6

Signal Transduction
β−Catenin (active) pERK1/2 p-p38 pStat1 pStat3 pAKT pS6 p4E-BP1

βlac-
βlac+
0 2 0 3 0 2 0 2.5 0 2 0 6 0 5 0 5

D.
Viral Parameters Cell Surface Proteins Cell Cycle & Survival
400 * 800 30 500 *** 300 *** 2.5 4 ***
* 20 250
*** *** ***
300 *** 400 2.0 3 200
600 15
20 200
300 1.5 150
200 400 2 10
200 1.0 100
10 100
100 200 1 5
100 0.5 50
0 0 0 0 0 0.0 0 0 0
vRCA pHistone H2AX Bst2 CD9 CD63 Ly6C MHCI Ki-67 pRb
Raw Median Expression

Signal Transduction
2.0 **** 10 2.5 ** 4 ** 3 15 **** 80 150
**** * *
****
1.5 8 2.0 3 60
6 1.5
2 10 100 Mock
1.0 2 40
4 1.0 βlac-
1 5 50
0.5 2 0.5 1 20 βlac+
0.0 0 0.0 0 0 0 0 0
β−Catenin (active) pERK1/2 p-p38 pStat1 pStat3 pAKT pS6 p4E-BP1