HEXANE

Conc. T1 T2 T3 T4 T5 T6 T7 T8 T9
2000 0.145 0.088 0.11 0.108
1000 0.062 0.091 0.089 0.069
100 0.063 0.073 0.063 0.058
10 0.061 0.058 0.061 0.057
1 0.076 0.062 0.115 0.055

BLANK

Conc. T1 T2 T3 T4 T5 T6 T7 T8 T9
2000 0.056 0.057 0.055 0.06
1000 0.056 0.057 0.055 0.06
100 0.056 0.057 0.055 0.06
10 0.056 0.057 0.055 0.06
1 0.056 0.057 0.055 0.06

HEXANE

Conc. T1 T2 T3 T4 T5 T6 T7 T8 T9
2000 0.852 0.372 1.074 0.823
1000 0.413 2.138 1.336 1.817
100 1.612 1.979 1.423 1.563
10 1.734 1.719 1.521 2.012
1 1.847 1.548 1.74 1.799

BLANK

Conc. T1 T2 T3 T4 T5 T6 T7 T8 T9
2000 1.756 1.504 1.597 1.638
1000 1.756 1.504 1.597 1.638
100 1.756 1.504 1.597 1.638
10 1.756 1.504 1.597 1.638
1 1.756 1.504 1.597 1.638

HEXANE
 HEXANE

Conc. T1 T2 T3 T4 T5 T6 T7 T8 T9
2000 0.707 0.284 0.964 0.715 0 0 0 0 0
1000 0.351 2.047 1.247 1.748 0 0 0 0 0
100 1.549 1.906 1.36 1.505 0 0 0 0 0
10 1.673 1.661 1.46 1.955 0 0 0 0 0
1 1.771 1.486 1.625 1.744 0 0 0 0 0

BLANK

Conc. T1 T2 T3 T4 T5 T6 T7 T8 T9
2000 1.7 1.447 1.542 1.578 0 0 0 0 0
1000 1.7 1.447 1.542 1.578 0 0 0 0 0
100 1.7 1.447 1.542 1.578 0 0 0 0 0
10 1.7 1.447 1.542 1.578 0 0 0 0 0
1 1.7 1.447 1.542 1.578 0 0 0 0 0

SELECTED CHANGE IN ABSORBANCE

HEXANE DICHLOROMETHANE

Conc. T1 T2 T3 T4 Conc. T1 T2 T3
2000 0.707 0.284 0.964 0.715 2000 0.856 1.56 1.201
1000 0.351 2.047 1.247 1.748 1000 1.67 1.557 1.672
100 1.549 1.906 1.36 1.505 100 1.719 1.733 1.842
10 1.673 1.661 1.46 1.955 10 1.668 1.593 1.9
1 1.771 1.486 1.625 1.744 1 1.526 1.583 2.02

BLANK BLANK

Conc. T1 T2 T3 T4 Conc. T1 T2 T3
2000 1.7 1.447 1.542 1.578 2000 1.7 1.447 1.542
1000 1.7 1.447 1.542 1.578 1000 1.7 1.447 1.542
100 1.7 1.447 1.542 1.578 100 1.7 1.447 1.542
10 1.7 1.447 1.542 1.578 10 1.7 1.447 1.542
1 1.7 1.447 1.542 1.578 1 1.7 1.447 1.542
 PRE-READING ABSOR

DICHLOROMETHA

T10 T11 T12 Conc. T1 T2 T3 T4 T5

2000 0.111 0.165 0.192 0.1
1000 0.089 0.079 0.098 0.099
100 0.065 0.084 0.066 0.075
10 0.06 0.082 0.056 0.06
1 0.061 0.056 0.072 0.078

BLANK

T10 T11 T12 Conc. T1 T2 T3 T4 T5

2000 0.056 0.057 0.055 0.06
1000 0.056 0.057 0.055 0.06
100 0.056 0.057 0.055 0.06
10 0.056 0.057 0.055 0.06
1 0.056 0.057 0.055 0.06

FINAL READING ABSOR

DICHLOROMETHA

T10 T11 T12 Conc. T1 T2 T3 T4 T5

2000 0.967 1.725 1.393 1.028
1000 1.759 1.636 1.77 1.568
100 1.784 1.817 1.908 1.654
10 1.728 1.675 1.956 1.569
1 1.587 1.639 2.092 2.244

BLANK

T10 T11 T12 Conc. T1 T2 T3 T4 T5

2000 1.756 1.504 1.597 1.638
1000 1.756 1.504 1.597 1.638
100 1.756 1.504 1.597 1.638
10 1.756 1.504 1.597 1.638
1 1.756 1.504 1.597 1.638

CHANGE IN ABSORB

DICHLOROMETHA
 DICHLOROMETHA

T10 T11 T12 Conc. T1 T2 T3 T4 T5

0 0 0 2000 0.856 1.56 1.201 0.928 0
0 0 0 1000 1.67 1.557 1.672 1.469 0
0 0 0 100 1.719 1.733 1.842 1.579 0
0 0 0 10 1.668 1.593 1.9 1.509 0
0 0 0 1 1.526 1.583 2.02 2.166 0

BLANK

T10 T11 T12 Conc. T1 T2 T3 T4 T5

0 0 0 2000 1.7 1.447 1.542 1.578 0
0 0 0 1000 1.7 1.447 1.542 1.578 0
0 0 0 100 1.7 1.447 1.542 1.578 0
0 0 0 10 1.7 1.447 1.542 1.578 0
0 0 0 1 1.7 1.447 1.542 1.578 0

BSORBANCE
HEXANE
HANE ACARBOSE

T4 Conc. T1 T2 T3 T4 Conc. T1
0.928 2000 1.625 1.647 1.488 1.382 2000 58.411765
1.469 1000 1.722 1.293 1.468 1.491 1000 79.352941
1.579 100 1.905 2.046 2.079 1.688 100 8.8823529
1.509 10 1.98 2.03 2.812 2.575 10 1.5882353
2.166 1 1.854 1.905 1.701 1.511 1 -4.176471

BLANK

T4 Conc. T1 T2 T3 T4
1.578 2000 1.7 1.447 1.542 1.578
1.578 1000 1.7 1.447 1.542 1.578
1.578 100 1.7 1.447 1.542 1.578
1.578 10 1.7 1.447 1.542 1.578
1.578 1 1.7 1.447 1.542 1.578
PRE-READING ABSORBANCE

DICHLOROMETHANE

T6 T7 T8 T9 T10 T11 T12 Conc. T1

2000 0.069
1000 0.058
100 0.055
10 0.062
1 0.072

BLANK

T6 T7 T8 T9 T10 T11 T12 Conc. T1

2000 0.056
1000 0.056
100 0.056
10 0.056
1 0.056

FINAL READING ABSORBANCE

DICHLOROMETHANE

T6 T7 T8 T9 T10 T11 T12 Conc. T1

2000 1.694
1000 1.78
100 1.96
10 2.042
1 1.926

BLANK

T6 T7 T8 T9 T10 T11 T12 Conc. T1

2000 1.756
1000 1.756
100 1.756
10 1.756
1 1.756

CHANGE IN ABSORBANCE

DICHLOROMETHANE
DICHLOROMETHANE

T6 T7 T8 T9 T10 T11 T12 Conc. T1

0 0 0 0 0 0 0 2000 1.625
0 0 0 0 0 0 0 1000 1.722
0 0 0 0 0 0 0 100 1.905
0 0 0 0 0 0 0 10 1.98
0 0 0 0 0 0 0 1 1.854

BLANK

T6 T7 T8 T9 T10 T11 T12 Conc. T1

0 0 0 0 0 0 0 2000 1.7
0 0 0 0 0 0 0 1000 1.7
0 0 0 0 0 0 0 100 1.7
0 0 0 0 0 0 0 10 1.7
0 0 0 0 0 0 0 1 1.7

%I
DICHLOROMETHANE

T2 T3 T4 MEAN SD %RSD Conc. T1

80.373186 37.483787 54.68948 58.756246 21.446774 36.501267 2000 49.647059
-41.4651 19.130999 -10.77313 19.00628 60.409117 317.83767 1000 1.7647059
-31.7208 11.802853 4.626109 -3.678532 24.32918 -661.3829 100 -1.117647
-14.78922 5.3177691 -23.891 -2.627738 10.69596 -407.0406 10 1.8823529
-2.695232 -5.38262 -10.51965 -4.084774 1.3460388 -32.95259 1 10.235294

%INHIBITION
80

60

40
%INHIBITION

20

0
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

-20

-40
%INH
0
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

-20

-40

-60

CONCENTRATION
 ACARBOSE

T2 T3 T4 T5 T6 T7 T8 T9 T10 T11
0.062 0.081 0.083
0.056 0.065 0.07
0.056 0.058 0.056
0.058 0.058 0.063
0.053 0.053 0.058

BLANK

T2 T3 T4 T5 T6 T7 T8 T9 T10 T11
0.057 0.055 0.06
0.057 0.055 0.06
0.057 0.055 0.06
0.057 0.055 0.06
0.057 0.055 0.06

ACARBOSE

T2 T3 T4 T5 T6 T7 T8 T9 T10 T11
1.709 1.569 1.465
1.349 1.533 1.561
2.102 2.137 1.744
2.088 2.87 2.638
1.958 1.754 1.569

BLANK

T2 T3 T4 T5 T6 T7 T8 T9 T10 T11
1.504 1.597 1.638
1.504 1.597 1.638
1.504 1.597 1.638
1.504 1.597 1.638
1.504 1.597 1.638

ACARBOSE
 ACARBOSE

T2 T3 T4 T5 T6 T7 T8 T9 T10 T11
1.647 1.488 1.382 0 0 0 0 0 0 0
1.293 1.468 1.491 0 0 0 0 0 0 0
2.046 2.079 1.688 0 0 0 0 0 0 0
2.03 2.812 2.575 0 0 0 0 0 0 0
1.905 1.701 1.511 0 0 0 0 0 0 0

BLANK

T2 T3 T4 T5 T6 T7 T8 T9 T10 T11
1.447 1.542 1.578 0 0 0 0 0 0 0
1.447 1.542 1.578 0 0 0 0 0 0 0
1.447 1.542 1.578 0 0 0 0 0 0 0
1.447 1.542 1.578 0 0 0 0 0 0 0
1.447 1.542 1.578 0 0 0 0 0 0 0

%INHIBITION
ACARBOSE

T2 T3 T4 MEAN SD %RSD Conc. T1 T2

-7.809261 22.114137 41.191381 21.317312 28.736446 134.80333 2000 4.4117647 -13.8217
-7.601935 -8.43061 6.9074778 -4.755946 5.6622304 -119.0558 1000 -1.294118 10.642709
-19.76503 -19.45525 -0.063371 -13.44598 10.67777 -79.41238 100 -12.05882 -41.39599
-10.08984 -23.2166 4.3726236 -10.4747 12.553902 -119.8498 10 -16.47059 -40.29026
-9.398756 -30.9987 -37.26236 -10.05405 20.624808 -205.1392 1 -9.058824 -31.65169

%INHIBITION

%INHIBITION (HEXANE)
DICHLOROMETHANE
ACARBOSE
100 1200 1300 1400 1500 1600 1700 1800 1900 2000 2100
 ACARBOSE
100 1200 1300 1400 1500 1600 1700 1800 1900 2000 2100

RATION
T12

T12

T12

T12
 T12
0
0
0
0
0

T12
0
0
0
0
0

T3 T4 MEAN SD %RSD
3.5019455 12.420786 -1.96933 10.274529 -521.7272
4.7989624 5.513308 4.7158513 5.9688473 126.56988
-34.8249 -6.970849 -29.42657 15.395578 -52.31863
-82.36057 -63.18124 -46.3738 33.363597 -71.94492
-10.31128 4.2458809 -17.00727 12.697897 -74.66159