tiempo temperatura 100

(min) 85 90 95 90
0 24.89 25.01 24.87 80
1 24.99 25.82 25
70
2 25.55 25.84 25.08
60
3 27.91 29.78 32.69
4 34.74 37.5 42.2 50
5 46.33 46.97 54.3 40
6 54.43 55.16 62.29 30
7 61.37 61.71 68.9 20
8 66.27 67.31 74.13
10
9 70.3 71.38 78.41
10 73.29 75.05 81.5 0
0 5 10 15 20 25 30 35
11 75.71 77.67 83.97
12 77.59 79.72 86.01 90 95 85

13 79.04 81.44 87.7

14 80.15 82.76 89.05
15 80.99 83.93 90.1
16 81.68 84.87 90.98
17 82.26 85.66 91.78
18 82.73 86.36 92.42
19 83.11 86.92 92.81
20 83.42 87.37 93.15
21 83.67 87.8 93.47
22 83.91 88.19 93.76
23 84.09 88.49 93.96
24 84.25 88.74 94.22
25 84.37 88.95 94.32
26 84.49 89.12 94.47
27 84.58 89.33 94.58
28 84.68 89.45 94.72
29 84.72 89.57 94.78
30 84.77 89.68 94.85
31 84.84 89.81 94.94
32 84.89 89.87 95.01
33 84.95 89.97 95.06
34 84.95 90.05 95.09
35 85 90.08 95.12
36 85.05 90.3 95.13
37 85.02 90.18 95.16
38 85.06 90.22 95.21
20 25 30 35 40

95 85
 Temperatura y tiempo de exposicion al tratamiento
termico de esporas bacillus Subtilis/amyloliquefaciens

muestras 0 85 C x 35 min90 C x 25 min95 C x 15 min

1 75000000 420000 16000 600
2 18000000 120000 13000 110
4 62000000 310000 48000 540
promedio 51666666.6667 283333.333 25666.6667 416.666667
1 7.87506126339 5.62324929 4.20411998 2.77815125
2 7.2552725051 5.07918125 4.11394335 2.04139269
3 7.7923916895 5.49136169 4.68124124 2.73239376
promedio 7.71321044345 5.45229767 4.40936947 2.61978876

temperatura tiempo log(UFC/mL) pendientes

0 7.71321044 0.0646 9
85 35 5.45229767 0.1322 8
0 7.71321044 0.3396 f(x) = − 0.0645975077844571
0.339561445677482 x x++7.7132104434
0.132153638919912 7.713210443
90 25 4.40936947 7 R² = 1
0 7.71321044 6
95 15 2.61978876
5
Temperatura(ºC) 4
85 90 95
Valor D(min) 15.479876161 7.56429652 2.94464075 3

0
0 5 10 15 20
39561445677482 x x++7.71321044345063
32153638919912
645975077844571 7.71321044345063

10 15 20 25 30 35 40