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TP N°04
Détermination des
phénotypes sanguins
Réalisée par:
• GHERMI Mohamed.
• CHAREF KHODJA Meriem.
• TAHRI Mahmoud.
• BETTAYEB Mohamed Amine.
• ZIDOUR Mehdi Nabil
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a- Système Rh (ISBT 004) :
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La protéine Rh comporte 6 boucles extracellulaires, 5 intracellulaires et 12 segments intramembranaires.
Les extrémités NH2 et COOH sont en position intracellulaire. Elle compte 417 acides aminés. En fonction
des allèles 34 (Ce) à 38 (cE) aa peuvent différer entre les protéines RhD et RhCE. Seul un nombre limité de
ces différences est en position extracellulaire. En cas d’allèle C ces différences sont limitées aux boucles 3,
4 et 6 qui portent l’antigénicité D (dont certains sont représentés sous forme de cercles noirs). En cas
d’allèle c la boucle 2 est aussi concernée. Les acides aminés considérés comme critiques pour les
spécificités C/c et E/e sont respectivement en position 103 et 226. Les 10 segments identifiés par un trait
gris correspondent aux différents exons. Le résidu Cys16 est habituellement, mais pas exclusivement
associé à l’antigène C et le résidu Trp16 est habituellement, mais pas exclusivement associé à l’antigène c
(74 % des sujets africains C+, c- possèdent un résidu Cys16).
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